Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605004857_nebe TTTATTTATCGATATCTCTTCTTTTCTGACTG(+ACTCAC)GATCCCTTCATCCCATCTTCCCAATACACAG NGG 10 8 17 8 24 0 100 5 10
    240605004854_b6yi CCATTTTTGTTCACAACCATTTCCCCTTCCTG(+ACTCAC)GATCCTCTGTCTGGTACCAAGCAATATATTT NGG 10 8 17 8 24 0 100 5 10
    240605004853_5zkp TAAAAAAATATATTGCTTGGTACCAGACAGAG(+ACTCAC)GATCCAGGAAGGGGAAATGGTTGTGAACAAA NGG 10 8 17 8 24 0 100 5 10
    240605004852_c09s CACCCTGGCTCTAGCACCATCTCCTGGGCTAG(+ACTCAC)GATCCTGGGCTAAATAAAAAGAAAGAAGACT NGG 10 8 17 8 24 0 100 5 10
    240605004849_k2pv TCCTAAGTCTTCTTTCTTTTTATTTAGCCCAG(+ACTCAC)GATCCTAGCCCAGGAGATGGTGCTAGAGCCA NGG 10 8 17 8 24 0 100 5 10
    240605004847_jf0z TCCTCAGAAAATTGGACATAGTACTACCAGAG(+ACTCAC)GATCCTGCAATACCTCTCCTGGGCATAGATC NGG 10 8 17 8 24 0 100 5 10
    240605004846_mtx4 TTCTGGATCTATGCCCAGGAGAGGTATTGCAG(+ACTCAC)GATCCTCTGGTAGTACTATGTCCAATTTTCT NGG 10 8 17 8 24 0 100 5 10
    240605004844_jh1k GGATGGACCATCTAGAGACTGCCATATCCACG(+ACTCAC)GATCCATCCCATAATTAGCCTCCAAACGATG NGG 10 8 17 8 24 0 100 5 10
    240605004842_czme GGTGTCATCGTTTGGAGGCTAATTATGGGATG(+ACTCAC)GATCCGTGGATATGGCAGTCTCTAGATGGTC NGG 10 8 17 8 24 0 100 5 10
    240605004840_l6lt CATCTTCATGAGTTGCTCTGTTATTTTGCAGG(+ACTCAC)GATCCTGGGCAGCTTTGGCATTATCATGCAC NGG 10 8 17 8 24 0 100 5 10