Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605004931_781u AATTCACCTACACAGACAGAGTGCATCCCTGG(+ACTCAC)GTACCAACAACAGAGCTTTCCAAATTGTGGT NGG 10 8 17 8 24 0 100 5 10
    240605004928_441i GGTCCTCCTGTCTTTGGGGTTCCAGGCCCATG(+ACTCAC)GTACCACACCCATCTTTCACATCAGTGCTTG NGG 10 8 17 8 24 0 100 5 10
    240605004926_vw27 CATCCCAAGCACTGATGTGAAAGATGGGTGTG(+ACTCAC)GTACCATGGGCCTGGAACCCCAAAGACAGGA NGG 10 8 17 8 24 0 100 5 10
    240605004925_dt1n GCAACCCAATAATACTATCATAATCCGGACAG(+ACTCAC)GATCCTCCCCGACTGTGGAGAGAGACCCTGG NGG 10 8 17 8 24 0 100 5 10
    240605004923_zxiz TTTCTCCAGGGTCTCTCTCCACAGTCGGGGAG(+ACTCAC)GATCCTGTCCGGATTATGATAGTATTATTGG NGG 10 8 17 8 24 0 100 5 10
    240605004922_z02q CACCAGCTTGACCCTCTCGATCTACAGACATG(+ACTCAC)GATCCGATCATTGCACACATTATAGGCTCCA NGG 10 8 17 8 24 0 100 5 10
    240605004921_hbos TCTTGTGGAGCCTATAATGTGTGCAATGATCG(+ACTCAC)GATCCATGTCTGTAGATCGAGAGGGTCAAGC NGG 10 8 17 8 24 0 100 5 10
    240605004919_43it GTCCCTCTGTCAGAATGAGCACACCTTACCTG(+ACTCAC)GATCCCAGATTTTCTGTACATGCGTTTGTTC NGG 10 8 17 8 24 0 100 5 10
    240605004917_7qfr ACAAAGAACAAACGCATGTACAGAAAATCTGG(+ACTCAC)GATCCAGGTAAGGTGTGCTCATTCTGACAGA NGG 10 8 17 8 24 0 100 5 10
    240605004915_i61e CTACTGTGGCTGACATCCAAAGGTATGGGGAG(+ACTCAC)GATCCTTGCTAGGAAGACCTCAGTCCTTTTA NGG 10 8 17 8 24 0 100 5 10