Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605003425_urt4 GCAGTGCCCTTAGAGGCCAGTAGAGTGCACAG(+ACTCAC)GATCCCCTGGAAATGGAGTGACAGCTTCGAG NGG 10 8 17 8 24 0 100 5 10
    240605003424_jt70 TGTTCCTCTTCCCTGCTGAGTATAGGTAGCTG(+ACTCAC)GATCCTGCGGTCCACTTTGCTTACTATCTAA NGG 10 8 17 8 24 0 100 5 10
    240605003421_198a GGCAGTTAGATAGTAAGCAAAGTGGACCGCAG(+ACTCAC)GATCCAGCTACCTATACTCAGCAGGGAAGAG NGG 10 8 17 8 24 0 100 5 10
    240605003420_uqgo GAAGTGCCTGTGGAGGTCAGACGAGGGTGTCG(+ACTCAC)GATCCCCTGGATGTGGAGTTGCAGGCAGTTG NGG 10 8 17 8 24 0 100 5 10
    240605003418_91ss GCTTACAACTGCCTGCAACTCCACATCCAGGG(+ACTCAC)GATCCGACACCCTCGTCTGACCTCCACAGGC NGG 10 8 17 8 24 0 100 5 10
    240605003416_3t1c CAGTGAAGAGCACACTGGCTGCTCTCCCTGAG(+ACTCAC)GATCCAAATTCAATTTTCAGCACCACATGGC NGG 10 8 17 8 24 0 100 5 10
    240605003415_pcmh AAGCTGCCATGTGGTGCTGAAAATTGAATTTG(+ACTCAC)GATCCTCAGGGAGAGCAGCCAGTGTGCTCTT NGG 10 8 17 8 24 0 100 5 10
    240605003414_nx0d CAGTTCGCTCCCCATGCAGCCATACCAGATAG(+ACTCAC)GTACCCTGGCTGGCTGTTAGAGAAGAGTTTC NGG 10 8 17 8 24 0 100 5 10
    240605003411_8azi CTGGGGAAACTCTTCTCTAACAGCCAGCCAGG(+ACTCAC)GTACCTATCTGGTATGGCTGCATGGGGAGCG NGG 10 8 17 8 24 0 100 5 10
    240605003410_zep7 CCAGAATCATATGACTGAGTTGGTAGAGCCAG(+ACTCAC)GTACCAGTGTGTGATCTTAAAAGCAAGCCAT NGG 10 8 17 8 24 0 100 5 10