Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605003408_jfll GCAGCATGGCTTGCTTTTAAGATCACACACTG(+ACTCAC)GTACCTGGCTCTACCAACTCAGTCATATGAT NGG 10 8 17 8 24 0 100 5 10
    240605003407_x3gm AGCATTATACCCAATTGATTACACTAAATCAG(+ACTCAC)GTACCAAAGTACTAGCTTTTCTGAAGGAGTC NGG 10 8 17 8 24 0 100 5 10
    240605003406_lyn1 ATTAAGACTCCTTCAGAAAAGCTAGTACTTTG(+ACTCAC)GTACCTGATTTAGTGTAATCAATTGGGTATA NGG 10 8 17 8 24 0 100 5 10
    240605003404_m8yl GGGAGAGGATGACTCTCTTGATGGTGTATGGG(+ACTCAC)GATCCACAACTTGAGGGTCATTTGGCAGCAC NGG 10 8 17 8 24 0 100 5 10
    240605003403_z909 CTATTGTGCTGCCAAATGACCCTCAAGTTGTG(+ACTCAC)GATCCCCATACACCATCAAGAGAGTCATCCT NGG 10 8 17 8 24 0 100 5 10
    240605003359_yejd ATGACTTCAGGCCTTTCCCAGCCTCCTCCCGG(+ACTCAC)GATCCAGGAAGGCAGCAGGATATTTGTGTGA NGG 10 8 17 8 24 0 100 5 10
    240605003357_bq6e CCACATCACACAAATATCCTGCTGCCTTCCTG(+ACTCAC)GATCCCGGGAGGAGGCTGGGAAAGGCCTGAA NGG 10 8 17 8 24 0 100 5 10
    240605003356_8krw GCAACTGACAGAAGTGTCAGCCTGGGCAGCAG(+ACTCAC)GATCCCTCGGTTCTGTGGTGTCCCCCAGGCA NGG 10 8 17 8 24 0 100 5 10
    240605003353_325a CGTGTTGCCTGGGGGACACCACAGAACCGAGG(+ACTCAC)GATCCTGCTGCCCAGGCTGACACTTCTGTCA NGG 10 8 17 8 24 0 100 5 10
    240605003351_pmmh GATTTGGAAGTGGGTGCACTGCCACACCTGTG(+ACTCAC)GTACCAGCACTTGAGAAGCTGAGGCAGTAAG NGG 10 8 17 8 24 0 100 5 10