Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605003301_j7mi TCCTAGAAGCTCTTCTCATTGTTACAGAAAAG(+ACTCAC)GTACCTCTAGCAATTTCCACGTCCCAAAAAT NGG 10 8 17 8 24 0 100 5 10
    240605003259_pn81 GGCATTTTCCTAATTAGTGATTGATAGGGGAG(+ACTCAC)GATCCAGCCATTTGTGGGTGGTGCCATCCCT NGG 10 8 17 8 24 0 100 5 10
    240605003257_6ty0 AGCCAAGGGATGGCACCACCCACAAATGGCTG(+ACTCAC)GATCCTCCCCTATCAATCACTAATTAGGAAA NGG 10 8 17 8 24 0 100 5 10
    240605003256_ovsu GCCACAGATGGTACATCTAGTGGTCCAGATAG(+ACTCAC)GATCCCTCCTGAAATCTCCTACTTCTTGTCT NGG 10 8 17 8 24 0 100 5 10
    240605003254_w8bx GTGGCAGACAAGAAGTAGGAGATTTCAGGAGG(+ACTCAC)GATCCTATCTGGACCACTAGATGTACCATCT NGG 10 8 17 8 24 0 100 5 10
    240605003253_f2ri TCTACCAAAAACAGGGCTAAGGGCTGGAGATG(+ACTCAC)GATCCCCGTTCCTTGTCCAGCCTCCTGCTAC NGG 10 8 17 8 24 0 100 5 10
    240605003251_qa8g CAGCGGTAGCAGGAGGCTGGACAAGGAACGGG(+ACTCAC)GATCCATCTCCAGCCCTTAGCCCTGTTTTTG NGG 10 8 17 8 24 0 100 5 10
    240605003248_z4li GTGCATATGCTGACGCCTGAGAACAGACCCAG(+ACTCAC)GTACCCGTGGCTGAGCTGAGCTTCCTCTGCT NGG 10 8 17 8 24 0 100 5 10
    240605003246_3xkj GGGAAAGCAGAGGAAGCTCAGCTCAGCCACGG(+ACTCAC)GTACCTGGGTCTGTTCTCAGGCGTCAGCATA NGG 10 8 17 8 24 0 100 5 10
    240605003245_agfx GACATGCCACCCACCGCACTTGGCTTTATCAG(+ACTCAC)GATCCATTTGTTGGTAGAAGCAAAAGCAGCT NGG 10 8 17 8 24 0 100 5 10