Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605003151_ozaw TTGCAGAGAGTTTGGATTTTGGAAGCTGCCAG(+ACTCAC)GTACCCATCACAGCAGGGGTGCCAGTGACAA NGG 10 8 17 8 24 0 100 5 10
    240605003150_ezrg CCTAAACAACAAAATTCATACATGAAAAAACG(+ACTCAC)GTACCACAGGTTTTGGTGTCCTCACTAACAG NGG 10 8 17 8 24 0 100 5 10
    240605003148_l3rc GACATCTGTTAGTGAGGACACCAAAACCTGTG(+ACTCAC)GTACCGTTTTTTCATGTATGAATTTTGTTGT NGG 10 8 17 8 24 0 100 5 10
    240605003145_0d3h CTGAAGATGATATTTTGATTTGTATTTTGGGG(+ACTCAC)GTACCTGTGTTGAGTCGATAAAACATTTTCA NGG 10 8 17 8 24 0 100 5 10
    240605003144_pmbz GGAGATGAAAATGTTTTATCGACTCAACACAG(+ACTCAC)GTACCCCCAAAATACAAATCAAAATATCATC NGG 10 8 17 8 24 0 100 5 10
    240605003142_vqti AACCTCTCCCTAGCAGGCTTCCCTGGGACGGG(+ACTCAC)GTACCTTTCCAGTTGGTGTTCCCTGGCTTGT NGG 10 8 17 8 24 0 100 5 10
    240605003141_8j50 CACACACAAGCCAGGGAACACCAACTGGAAAG(+ACTCAC)GTACCCCGTCCCAGGGAAGCCTGCTAGGGAG NGG 10 8 17 8 24 0 100 5 10
    240605003139_lcj1 CGTGTGTTCCTGCCAGCATAGGACCAGGATGG(+ACTCAC)GTACCTTCTCCAAGCTCCGCAGAGGAGGACC NGG 10 8 17 8 24 0 100 5 10
    240605003138_7fwp GCTGGGGTCCTCCTCTGCGGAGCTTGGAGAAG(+ACTCAC)GTACCCATCCTGGTCCTATGCTGGCAGGAAC NGG 10 8 17 8 24 0 100 5 10
    240605003135_ycl0 CCCAAATCAAAAGAAAATTCACATTTGCCCTG(+ACTCAC)GATCCCAGACATAGACATATACAGTCATACA NGG 10 8 17 8 24 0 100 5 10