Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605003209_nw8r TAAGGTAACCTGGCTTCTGTGGAAGAAGAAGG(+ACTCAC)GTACCAGGCTGCCCTTATACTAGATCTGCGG NGG 10 8 17 8 24 0 100 5 10
    240605003208_htle TCCCAGAATAAACAATCCAGGAAGGCCAGCTG(+ACTCAC)GATCCAAGGGACTCAACGACCTCTCCAGGCA NGG 10 8 17 8 24 0 100 5 10
    240605003205_pz71 GGCTCTGCCTGGAGAGGTCGTTGAGTCCCTTG(+ACTCAC)GATCCAGCTGGCCTTCCTGGATTGTTTATTC NGG 10 8 17 8 24 0 100 5 10
    240605003204_4hkt AGCCCTGTTAGGGAGCTTGAGACAGGGGTCTG(+ACTCAC)GATCCTCGGGAGACCACTGGGTATAATCTAT NGG 10 8 17 8 24 0 100 5 10
    240605003201_7wat GAAAAATAGATTATACCCAGTGGTCTCCCGAG(+ACTCAC)GATCCAGACCCCTGTCTCAAGCTCCCTAACA NGG 10 8 17 8 24 0 100 5 10
    240605003159_40me TGTTTCATTTGAAGTATGGAGCTCTAAAAGGG(+ACTCAC)GATCCTGTGAGAGGCCTCGGGTTACTAGATG NGG 10 8 17 8 24 0 100 5 10
    240605003158_c78w ACAAGCATCTAGTAACCCGAGGCCTCTCACAG(+ACTCAC)GATCCCCTTTTAGAGCTCCATACTTCAAATG NGG 10 8 17 8 24 0 100 5 10
    240605003156_ki8e AAAACTCATGCATAAAACAACAGGTTTCCTTG(+ACTCAC)GATCCACAGCCTACCCTAAAATATGGAAAGA NGG 10 8 17 8 24 0 100 5 10
    240605003155_3s31 CTATTTCTTTCCATATTTTAGGGTAGGCTGTG(+ACTCAC)GATCCAAGGAAACCTGTTGTTTTATGCATGA NGG 10 8 17 8 24 0 100 5 10
    240605003153_ghv2 CATCCTTGTCACTGGCACCCCTGCTGTGATGG(+ACTCAC)GTACCTGGCAGCTTCCAAAATCCAAACTCTC NGG 10 8 17 8 24 0 100 5 10