Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605003227_xzvg TCATTACTTTGCAAACCAGATGCTGTGGCCTG(+ACTCAC)GTACCTGCCACAAAGGCCATGTGGGCACAGG NGG 10 8 17 8 24 0 100 5 10
    240605003225_v5bh GGAGAGAGAAAGCCGCTCTTTGGGGGCACCTG(+ACTCAC)GATCCACTCACAGCTGTCTCCTGCCCGTGGG NGG 10 8 17 8 24 0 100 5 10
    240605003224_fmm3 CTGGTCCCACGGGCAGGAGACAGCTGTGAGTG(+ACTCAC)GATCCAGGTGCCCCCAAAGAGCGGCTTTCTC NGG 10 8 17 8 24 0 100 5 10
    240605003222_b0qx GAAAGGAGGGGGGCCACAGGCAAGAAGACAGG(+ACTCAC)GTACCCACGCATAGACTTTCCATCTCCCAAC NGG 10 8 17 8 24 0 100 5 10
    240605003220_8jkw GCGTTGTTGGGAGATGGAAAGTCTATGCGTGG(+ACTCAC)GTACCCTGTCTTCTTGCCTGTGGCCCCCCTC NGG 10 8 17 8 24 0 100 5 10
    240605003218_o6ly GCCAAGTGGTATTTTGGAAACAGGGACATCGG(+ACTCAC)GTACCCTACAGAGTGTAGACAAACACGTTTT NGG 10 8 17 8 24 0 100 5 10
    240605003217_ocmb GGGTCAAAACGTGTTTGTCTACACTCTGTAGG(+ACTCAC)GTACCCGATGTCCCTGTTTCCAAAATACCAC NGG 10 8 17 8 24 0 100 5 10
    240605003215_mvnu GCCAAGTGGTATTTTGGAAACAGGGACATCGG(+ACTCAC)GTACCCTACAGAGTGTAGACAAACACGTTTT NGG 10 8 17 8 24 0 100 5 10
    240605003213_laso GGGTCAAAACGTGTTTGTCTACACTCTGTAGG(+ACTCAC)GTACCCGATGTCCCTGTTTCCAAAATACCAC NGG 10 8 17 8 24 0 100 5 10
    240605003211_0wk1 TCCCTCCGCAGATCTAGTATAAGGGCAGCCTG(+ACTCAC)GTACCCTTCTTCTTCCACAGAAGCCAGGTTA NGG 10 8 17 8 24 0 100 5 10