Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613161246_m6yw GCTTCAACCAAGACAGGGCACCCCAAGCCAGG(+AGGACA)GTACCCCAGCAGCCCCCCTGGAGAACAAGGC NGG 10 8 17 8 24 0 100 5 10
    240613161244_hzim AGTGCAGTCCAGGCTAGCCTTGATCTTAACGG(+AGGACA)GATCCCTGTGCTGACTCAGCCTTTTTTTTTT NGG 10 8 17 8 24 0 100 5 10
    240613161243_aj9m AAAAAAAAAAAAAAAGGCTGAGTCAGCACAGG(+AGGACA)GATCCCGTTAAGATCAAGGCTAGCCTGGACT NGG 10 8 17 8 24 0 100 5 10
    240613161241_upz1 GCCAGGGACTTTTCTCCTTCTCACTTGGCCAG(+AGGACA)GTACCCCTAACTCCTGCTTGCAGAGGAATCC NGG 10 8 17 8 24 0 100 5 10
    240613161238_bnr6 TCATTGGATTCCTCTGCAAGCAGGAGTTAGGG(+AGGACA)GTACCTGGCCAAGTGAGAAGGAGAAAAGTCC NGG 10 8 17 8 24 0 100 5 10
    240613161237_4t63 TATGAGACCTGACAGTTGAATGGGTGGCGTAG(+AGGACA)GATCCGTGAAAATTTTGGGCAGTCTTGTGGA NGG 10 8 17 8 24 0 100 5 10
    240613161236_3fbz CGGGCTCCACAAGACTGCCCAAAATTTTCACG(+AGGACA)GATCCTACGCCACCCATTCAACTGTCAGGTC NGG 10 8 17 8 24 0 100 5 10
    240613161234_1omr CAAGAGGCCAGCCACTGAAGCCAGTGGCCTGG(+AGGACA)GTACCCAGGGGCCACTCCTTCCTCATAGACT NGG 10 8 17 8 24 0 100 5 10
    240613161231_k9e9 GGCCCAGTCTATGAGGAAGGAGTGGCCCCTGG(+AGGACA)GTACCCAGGCCACTGGCTTCAGTGGCTGGCC NGG 10 8 17 8 24 0 100 5 10
    240613161230_n0up AAGGAACTTCTTAGATGGGCTTGTGATGTCAG(+AGGACA)GTACCCCCCCACCCTTACAGAGCGCAGACAT NGG 10 8 17 8 24 0 100 5 10