Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613161317_nc3c AAGATTCATGCTTCCATACCATGCAAAGAAGG(+AGGACA)GTACCCTACTATGGTACTGTCACCTTAAGGA NGG 10 8 17 8 24 0 100 5 10
    240613161316_3w0v CGCTTTGTCCTTATGTGAATCTGCTATTGCGG(+AGGACA)GATCCAAGCATTTGAGGAATATGTATATAGA NGG 10 8 17 8 24 0 100 5 10
    240613161315_jxv7 CATGTTCTATATACATATTCCTCAAATGCTTG(+AGGACA)GATCCCGCAATAGCAGATTCACATAAGGACA NGG 10 8 17 8 24 0 100 5 10
    240613161313_qaah GTAGTGCCTGCAGATGCCAGAAGAGGGCGATG(+AGGACA)GATCCTTTGGAAACAATGTTATTGGCAGCTT NGG 10 8 17 8 24 0 100 5 10
    240613161311_q5p8 AACTCAAGCTGCCAATAACATTGTTTCCAAAG(+AGGACA)GATCCATCGCCCTCTTCTGGCATCTGCAGGC NGG 10 8 17 8 24 0 100 5 10
    240613161310_viaa TGCTATGTAGTCCCAGCTGGCCTCAAACTCAG(+AGGACA)GATCCTCCTGCCTCAGCCTAGAATGGAACTC NGG 10 8 17 8 24 0 100 5 10
    240613161309_7jnh CTTAAGAGTTCCATTCTAGGCTGAGGCAGGAG(+AGGACA)GATCCTGAGTTTGAGGCCAGCTGGGACTACA NGG 10 8 17 8 24 0 100 5 10
    240613161307_vlve ATTTCAGTTTAGGAAGTGTGCCGTTTCATTAG(+AGGACA)GTACCAAAGCACTTGCACTTTTGGGAACATA NGG 10 8 17 8 24 0 100 5 10
    240613161306_h8rr ACACCTATGTTCCCAAAAGTGCAAGTGCTTTG(+AGGACA)GTACCTAATGAAACGGCACACTTCCTAAACT NGG 10 8 17 8 24 0 100 5 10
    240613161304_37hl AGAAATTGCTGCACCAAGTCAATTGGCCGCTG(+AGGACA)GATCCGGATGTATCAGGCTGGAAACATGACT NGG 10 8 17 8 24 0 100 5 10