Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613161419_ualw ATTTACACAGCATCCCTGAAGCAGCCTGCCTG(+AGGACA)GTACCTTCCAAGGTACATTGGCTATAAGTGG NGG 10 8 17 8 24 0 100 5 10
    240613161418_k3o9 CCCGACCTATTTTGGTTTTGGGGGGCATACGG(+AGGACA)GTACCTTTTGTGTTATTAGTTTATGTATATT NGG 10 8 17 8 24 0 100 5 10
    240613161417_n6xe ATCTTAATATACATAAACTAATAACACAAAAG(+AGGACA)GTACCCGTATGCCCCCCAAAACCAAAATAGG NGG 10 8 17 8 24 0 100 5 10
    240613161415_mfx8 TTTCAGTGGTATGTCAAGTACAAGGCCTCTGG(+AGGACA)GATCCACACAAGCAGATGTTCTTGGAAATCA NGG 10 8 17 8 24 0 100 5 10
    240613161414_k05r ATGGATGATTTCCAAGAACATCTGCTTGTGTG(+AGGACA)GATCCCAGAGGCCTTGTACTTGACATACCAC NGG 10 8 17 8 24 0 100 5 10
    240613161412_oldo AGGCCCAGGATCTAGATTGCAGAGAACTTGAG(+AGGACA)GATCCATGACTTAGCCAGCAGGTGTGTTTTG NGG 10 8 17 8 24 0 100 5 10
    240613161411_lmz6 TGGAGCAAAACACACCTGCTGGCTAAGTCATG(+AGGACA)GATCCTCAAGTTCTCTGCAATCTAGATCCTG NGG 10 8 17 8 24 0 100 5 10
    240613161409_ap88 GGCCCTGCTCAGATGGATGCAGAGCATTGAGG(+AGGACA)GATCCAGATGAGGTGTGGTCCAGGGAAGAGG NGG 10 8 17 8 24 0 100 5 10
    240613161408_i9n9 GATGCCCTCTTCCCTGGACCACACCTCATCTG(+AGGACA)GATCCCTCAATGCTCTGCATCCATCTGAGCA NGG 10 8 17 8 24 0 100 5 10
    240613161407_rhg4 CCTAACCTTCCTTACCACACCAGCCTGGGACG(+AGGACA)GATCCGAGCCCCAGCGGGAAGCCATTCTCTT NGG 10 8 17 8 24 0 100 5 10