Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613161507_5a61 AGCCGGACTCTCCACCCCCGACCTCCCCTCAG(+AGGACA)GATCCTGGGCACTGGCATTTTGGGGAAAGCC NGG 10 8 17 8 24 0 100 5 10
    240613161506_r3lq CAGCCATCTGCTGAGTGTTAGTTGAGAAGATG(+AGGACA)GATCCTAAGCCAGCTGAAGAATGAGTGCAGG NGG 10 8 17 8 24 0 100 5 10
    240613161504_io3b CACAGCCTGCACTCATTCTTCAGCTGGCTTAG(+AGGACA)GATCCATCTTCTCAACTAACACTCAGCAGAT NGG 10 8 17 8 24 0 100 5 10
    240613161502_r2wo CCAAACGTGGGCTGCTGCAATCCCTCAGCCTG(+AGGACA)GATCCTGAGCTACAGAAAAGAAGGCGGTCAA NGG 10 8 17 8 24 0 100 5 10
    240613161501_0c0h GCCAGTTGACCGCCTTCTTTTCTGTAGCTCAG(+AGGACA)GATCCAGGCTGAGGGATTGCAGCAGCCCACG NGG 10 8 17 8 24 0 100 5 10
    240613161459_okuc CATGGGAGAGGCCAGTCCACCTGACATGCCAG(+AGGACA)GATCCAAGAAGCAGTACTTTTAATTGGGTGA NGG 10 8 17 8 24 0 100 5 10
    240613161458_1wcd GGGTTTCACCCAATTAAAAGTACTGCTTCTTG(+AGGACA)GATCCTGGCATGTCAGGTGGACTGGCCTCTC NGG 10 8 17 8 24 0 100 5 10
    240613161457_3896 TCAGCTGGTGCAGGGTGAGAGAGCCAGAAATG(+AGGACA)GTACCACACCTGCCTTCCCACCTGGCTAACT NGG 10 8 17 8 24 0 100 5 10
    240613161455_rfws TGGCCAGTTAGCCAGGTGGGAAGGCAGGTGTG(+AGGACA)GTACCATTTCTGGCTCTCTCACCCTGCACCA NGG 10 8 17 8 24 0 100 5 10
    240613161454_6397 CCTGGGAGGGAGCCTTTCCTGGCTGTTTCATG(+AGGACA)GATCCACCACAGGATGTTTTTTAAGCAATTT NGG 10 8 17 8 24 0 100 5 10