Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613161524_6mws CTGAGCCTGACAACCTGTGTTTGGTCCTTGGG(+AGGACA)GATCCATATGGAAGGAAACAACCAACTCCCC NGG 10 8 17 8 24 0 100 5 10
    240613161523_a82t CCTGAGTGAGGACGTGGGGAGACAGGCACGAG(+AGGACA)GATCCCCAAGCTTGGCTTTGGGGAAAGAGTT NGG 10 8 17 8 24 0 100 5 10
    240613161521_a1qi GCCCCAACTCTTTCCCCAAAGCCAAGCTTGGG(+AGGACA)GATCCTCGTGCCTGTCTCCCCACGTCCTCAC NGG 10 8 17 8 24 0 100 5 10
    240613161519_7mtl GTCTTTAACTGCCTGTAAATGCAGTTCGCAGG(+AGGACA)GATCCAATCTCCATCTGACTTCTCTGAGTTC NGG 10 8 17 8 24 0 100 5 10
    240613161518_8rlt CTACAGAACTCAGAGAAGTCAGATGGAGATTG(+AGGACA)GATCCCTGCGAACTGCATTTACAGGCAGTTA NGG 10 8 17 8 24 0 100 5 10
    240613161517_s7ao CCAAGAATCACATATATCCGAACATGCTGGTG(+AGGACA)GTACCCTTTGGAAAAACTCCCATTTGGGCCT NGG 10 8 17 8 24 0 100 5 10
    240613161515_7oh9 TTTAAAGGCCCAAATGGGAGTTTTTCCAAAGG(+AGGACA)GTACCACCAGCATGTTCGGATATATGTGATT NGG 10 8 17 8 24 0 100 5 10
    240613161513_o22o TGGTCCCCCCAGGCTACTGGGAGGTGGGGCTG(+AGGACA)GATCCCTGGATGCTGAATCTAGCAGAGGGGA NGG 10 8 17 8 24 0 100 5 10
    240613161511_ec50 GGGATTCCCCTCTGCTAGATTCAGCATCCAGG(+AGGACA)GATCCAGCCCCACCTCCCAGTAGCCTGGGGG NGG 10 8 17 8 24 0 100 5 10
    240613161509_sjcs TGAAAGGCTTTCCCCAAAATGCCAGTGCCCAG(+AGGACA)GATCCTGAGGGGAGGTCGGGGGTGGAGAGTC NGG 10 8 17 8 24 0 100 5 10