Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613161545_by68 CCCACCTGTCTGCCCATCATCACTAGGGGCGG(+AGGACA)GTACCTACTGAAGAGGGAGGGCTGTTGAAAG NGG 10 8 17 8 24 0 100 5 10
    240613161543_9wfr GGTGTAGCCCTACCTACAGTGTGACTAGGCCG(+AGGACA)GTACCGATCCTGCCTGGTTCTAACCAGAAAA NGG 10 8 17 8 24 0 100 5 10
    240613161541_0not GTCTGTTTTCTGGTTAGAACCAGGCAGGATCG(+AGGACA)GTACCGGCCTAGTCACACTGTAGGTAGGGCT NGG 10 8 17 8 24 0 100 5 10
    240613161539_ngsf AACCCCTTTCAACAGCCCTCCCTCTTCAGTAG(+AGGACA)GTACCCGCCCCTAGTGATGATGGGCAGACAG NGG 10 8 17 8 24 0 100 5 10
    240613161537_3tq8 CCCACCTGTCTGCCCATCATCACTAGGGGCGG(+AGGACA)GTACCTACTGAAGAGGGAGGGCTGTTGAAAG NGG 10 8 17 8 24 0 100 5 10
    240613161536_2691 AACCTGAAGACCAAAGAGAAGGCAGCCAAGTG(+AGGACA)GATCCTGGCAGCTGTGGCGCTGCTCCTGCAG NGG 10 8 17 8 24 0 100 5 10
    240613161534_i4wj TACACCTGCAGGAGCAGCGCCACAGCTGCCAG(+AGGACA)GATCCACTTGGCTGCCTTCTCTTTGGTCTTC NGG 10 8 17 8 24 0 100 5 10
    240613161533_5ghs GACAGACAGACAAGGCTGCACGCTCCCTCAAG(+AGGACA)GATCCCGTCAGGACACTCCTGGCCTACTTTG NGG 10 8 17 8 24 0 100 5 10
    240613161527_ao8q GAATTCAAAGTAGGCCAGGAGTGTCCTGACGG(+AGGACA)GATCCTTGAGGGAGCGTGCAGCCTTGTCTGT NGG 10 8 17 8 24 0 100 5 10
    240613161526_d4k2 ATTTGGGGGAGTTGGTTGTTTCCTTCCATATG(+AGGACA)GATCCCCAAGGACCAAACACAGGTTGTCAGG NGG 10 8 17 8 24 0 100 5 10