Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613161638_ftpm CAGGATGAGAACCAGGATCAGCCTGCGGGCCG(+AGGACA)GTACCGGCCTGCCCCACTCTACAGCCCAGGA NGG 10 8 17 8 24 0 100 5 10
    240613161636_vk3n CCCACACCCTGGTGGCTAGAGGAGAGTAGGGG(+AGGACA)GTACCCTACAGCCACTGGGGGCTCCTGCCTG NGG 10 8 17 8 24 0 100 5 10
    240613161634_lm5v GCCTGCAGGCAGGAGCCCCCAGTGGCTGTAGG(+AGGACA)GTACCCCCTACTCTCCTCTAGCCACCAGGGT NGG 10 8 17 8 24 0 100 5 10
    240613161632_mejx CTTGAGGCAGCCACCTCACCAGGAACACCTGG(+AGGACA)GATCCAGCCCAGGCTGGGCCGATGTGAGGCG NGG 10 8 17 8 24 0 100 5 10
    240613161631_ckqw AGCCTCGCCTCACATCGGCCCAGCCTGGGCTG(+AGGACA)GATCCCAGGTGTTCCTGGTGAGGTGGCTGCC NGG 10 8 17 8 24 0 100 5 10
    240613161628_fs5c CAGCTGCCTAAAGCTGGGCCTGGCTGGCCGGG(+AGGACA)GATCCACTTAGTCCAGAAGGAGAAAGAGACA NGG 10 8 17 8 24 0 100 5 10
    240613161627_8bt5 ACCTTTGTCTCTTTCTCCTTCTGGACTAAGTG(+AGGACA)GATCCCCGGCCAGCCAGGCCCAGCTTTAGGC NGG 10 8 17 8 24 0 100 5 10
    240613161626_t6un GTTCTGCTTGCCCAGGACAGCTCAGCCTGTAG(+AGGACA)GATCCTCTCACAGGGAAGATGCTCTTGGCTA NGG 10 8 17 8 24 0 100 5 10
    240613161625_8d0p TGTTTTAGCCAAGAGCATCTTCCCTGTGAGAG(+AGGACA)GATCCTACAGGCTGAGCTGTCCTGGGCAAGC NGG 10 8 17 8 24 0 100 5 10
    240613161623_twew TCTAACATCTAGGTTGAGAACAAGCCTGAATG(+AGGACA)GTACCCATGGTTGGGAGAGAGAACTTGAACT NGG 10 8 17 8 24 0 100 5 10