Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613161729_010b AAAGGAAGGGTTTCCACTGAGGTACTTAGGAG(+AGGACA)GATCCCACCTCTCCAGGTATCGTGACAACTG NGG 10 8 17 8 24 0 100 5 10
    240613161728_s2r9 CATCTCCAGATACACAGTATGTCCAGTTGGTG(+AGGACA)GTACCTGAGCCCACCCTTGACTAGGGCAGCG NGG 10 8 17 8 24 0 100 5 10
    240613161726_4zg7 CAGGCCGCTGCCCTAGTCAAGGGTGGGCTCAG(+AGGACA)GTACCACCAACTGGACATACTGTGTATCTGG NGG 10 8 17 8 24 0 100 5 10
    240613161725_6gsp CCATGTGGGCTCCATGGATCAAACTAATGGTG(+AGGACA)GTACCAATAAGCCATCTTGCTGGCCCTAGAA NGG 10 8 17 8 24 0 100 5 10
    240613161723_a8yi TTGAATTCTAGGGCCAGCAAGATGGCTTATTG(+AGGACA)GTACCACCATTAGTTTGATCCATGGAGCCCA NGG 10 8 17 8 24 0 100 5 10
    240613161722_6lem GCCTGGGCCATGGCCTCTTCTAAGAAAAGTTG(+AGGACA)GTACCAACCACTAATGGTCCTTTTCTGCTCT NGG 10 8 17 8 24 0 100 5 10
    240613161721_ob7e CTAGAAGAGCAGAAAAGGACCATTAGTGGTTG(+AGGACA)GTACCAACTTTTCTTAGAAGAGGCCATGGCC NGG 10 8 17 8 24 0 100 5 10
    240613161719_9qsj TCCTGGCATCCTGCAGCCTTTAAGACCTGGGG(+AGGACA)GATCCCTTACAGAAACAGTCTTAGCCTTTCG NGG 10 8 17 8 24 0 100 5 10
    240613161717_bdpf AGGCTCGAAAGGCTAAGACTGTTTCTGTAAGG(+AGGACA)GATCCCCCAGGTCTTAAAGGCTGCAGGATGC NGG 10 8 17 8 24 0 100 5 10
    240613161716_4q1f ACTGCATTGGTAGGGTACAAAGGTGTTCAGAG(+AGGACA)GATCCCAGTCTGAGTGATTAGCCTAAGACTG NGG 10 8 17 8 24 0 100 5 10