Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613161831_9ivy CTTTGGACCTAGATGGCCTATTGCGGCTGGAG(+AGGACA)GATCCTGAGGACAGGAGAGGGGCTTCGGCTG NGG 10 8 17 8 24 0 100 5 10
    240613161829_9oa9 CCATTTCTATAAAGCTTGCTCCTTTGACACAG(+AGGACA)GTACCACCTGGTTTCACAAAGGAATGTGCTA NGG 10 8 17 8 24 0 100 5 10
    240613161828_zaqq GCAACTAGCACATTCCTTTGTGAAACCAGGTG(+AGGACA)GTACCTGTGTCAAAGGAGCAAGCTTTATAGA NGG 10 8 17 8 24 0 100 5 10
    240613161826_4wex CTGGAACAATTGACTCTGGTGAGTCTGAGTGG(+AGGACA)GTACCTATCTATTTCTGGCAATTAGATGAAA NGG 10 8 17 8 24 0 100 5 10
    240613161825_md6i ACAAATTTCATCTAATTGCCAGAAATAGATAG(+AGGACA)GTACCCACTCAGACTCACCAGAGTCAATTGT NGG 10 8 17 8 24 0 100 5 10
    240613161824_lu5s CTTTCTCCTCTTCCTTCTATTTGTCAAAGCTG(+AGGACA)GATCCAAGGCATCATGTATACTTAGGCAAAC NGG 10 8 17 8 24 0 100 5 10
    240613161822_m0xf ATTATGTTTGCCTAAGTATACATGATGCCTTG(+AGGACA)GATCCAGCTTTGACAAATAGAAGGAAGAGGA NGG 10 8 17 8 24 0 100 5 10
    240613161821_9y37 CTGGGCCAACCCCCAGCGAACCTCCGGCAGAG(+AGGACA)GATCCAGGGTGTGCAGTGGGGTCACTTTTGC NGG 10 8 17 8 24 0 100 5 10
    240613161819_pjc0 TTATGGCAAAAGTGACCCCACTGCACACCCTG(+AGGACA)GATCCTCTGCCGGAGGTTCGCTGGGGGTTGG NGG 10 8 17 8 24 0 100 5 10
    240613161817_1ay9 GGTCTGTCATGGCACGCCACACTGACTGGTGG(+AGGACA)GATCCTTACAGGAAACTCCAATCCCAGTGTG NGG 10 8 17 8 24 0 100 5 10