Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613161847_ezei TTAATTTTTGAGAATTGGTTTACCCAACTTAG(+AGGACA)GATCCATTCACATACTCACATTACCTGCTCA NGG 10 8 17 8 24 0 100 5 10
    240613161845_yyod GGATGAGAAGTCTGAACTCTACTGCACAACTG(+AGGACA)GATCCCTAGATTGAGCAGGCTTTATCTTTCA NGG 10 8 17 8 24 0 100 5 10
    240613161844_dfu9 GCTTTTGAAAGATAAAGCCTGCTCAATCTAGG(+AGGACA)GATCCAGTTGTGCAGTAGAGTTCAGACTTCT NGG 10 8 17 8 24 0 100 5 10
    240613161842_95kg CTGATGCCCATAGAGATCAGAAGAGGGCACTG(+AGGACA)GATCCCATGCATCGGGAGTATTGGATAGTTG NGG 10 8 17 8 24 0 100 5 10
    240613161841_iqyo GCCCACAACTATCCAATACTCCCGATGCATGG(+AGGACA)GATCCAGTGCCCTCTTCTGATCTCTATGGGC NGG 10 8 17 8 24 0 100 5 10
    240613161839_pu7p TCAACTTCCGGGAGGAAGGATGACTAGAGATG(+AGGACA)GTACCAAAGGTGGGAGACGCTTGGGCACCCT NGG 10 8 17 8 24 0 100 5 10
    240613161837_ajw6 TGGCAAGGGTGCCCAAGCGTCTCCCACCTTTG(+AGGACA)GTACCATCTCTAGTCATCCTTCCTCCCGGAA NGG 10 8 17 8 24 0 100 5 10
    240613161836_t4ex AAATAAATCAGAGGAACCCTGGACCCAGCCAG(+AGGACA)GATCCTACCCTTTGGGGGATTGGAGGCGTGC NGG 10 8 17 8 24 0 100 5 10
    240613161834_q315 AGGCTGCACGCCTCCAATCCCCCAAAGGGTAG(+AGGACA)GATCCTGGCTGGGTCCAGGGTTCCTCTGATT NGG 10 8 17 8 24 0 100 5 10
    240613161832_ri89 CTGGTCAGCCGAAGCCCCTCTCCTGTCCTCAG(+AGGACA)GATCCTCCAGCCGCAATAGGCCATCTAGGTC NGG 10 8 17 8 24 0 100 5 10