Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613161901_ta4h GCTCATTTCCATGAGCAACTTTGAAATGTATG(+AGGACA)GTACCCATATAGTATCCTAACACGTGACCTT NGG 10 8 17 8 24 0 100 5 10
    240613161859_gqj6 TTATACCAGTGCCCGGGAGATGAGGATGGGTG(+AGGACA)GATCCGTGGTGCTGGCTAGACAGACAGCCCA NGG 10 8 17 8 24 0 100 5 10
    240613161857_nhwb TCTACTGGGCTGTCTGTCTAGCCAGCACCACG(+AGGACA)GATCCACCCATCCTCATCTCCCGGGCACTGG NGG 10 8 17 8 24 0 100 5 10
    240613161856_6amr AACTGAGCTCAAGGCCCAGGGACACATAGCAG(+AGGACA)GTACCAGGAGCAAGAGAGAGCCCCAGAAAGG NGG 10 8 17 8 24 0 100 5 10
    240613161854_85q8 GAACTCCTTTCTGGGGCTCTCTCTTGCTCCTG(+AGGACA)GTACCTGCTATGTGTCCCTGGGCCTTGAGCT NGG 10 8 17 8 24 0 100 5 10
    240613161853_1iil TGTGTATGTGTAGGTGAGAGAACGATTTTCAG(+AGGACA)GATCCTATCCACTCTTCCCTCCATGTAGGAC NGG 10 8 17 8 24 0 100 5 10
    240613161852_xdal ACCTGGTCCTACATGGAGGGAAGAGTGGATAG(+AGGACA)GATCCTGAAAATCGTTCTCTCACCTACACAT NGG 10 8 17 8 24 0 100 5 10
    240613161851_cr7a TCATTCTCACACAACCCCCTATCTATGGAATG(+AGGACA)GTACCATCCACAGTGGGCTGGGTCTTTCTAG NGG 10 8 17 8 24 0 100 5 10
    240613161849_h4ll TTTGCCTAGAAAGACCCAGCCCACTGTGGATG(+AGGACA)GTACCATTCCATAGATAGGGGGTTGTGTGAG NGG 10 8 17 8 24 0 100 5 10
    240613161848_unbr CCAACTGAGCAGGTAATGTGAGTATGTGAATG(+AGGACA)GATCCTAAGTTGGGTAAACCAATTCTCAAAA NGG 10 8 17 8 24 0 100 5 10