Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613161801_6vtn GCAGCAGAGTAACAAGGCAAGGGGGTAACATG(+AGGACA)GTACCAATTAGCCTCGGAGCAGAGCAGAGAA NGG 10 8 17 8 24 0 100 5 10
    240613161759_6vvk GTCTGCCGAGTTCCTATCATTTCCACTCCCAG(+AGGACA)GATCCATGGTATTCAGATCAAGTAAGGCTGT NGG 10 8 17 8 24 0 100 5 10
    240613161757_to1d CCTCCACAGCCTTACTTGATCTGAATACCATG(+AGGACA)GATCCTGGGAGTGGAAATGATAGGAACTCGG NGG 10 8 17 8 24 0 100 5 10
    240613161755_or5y GCTTCTGCAAATTATCGAAGCTTCTGAGGAAG(+AGGACA)GATCCATCCAGAGGCTCTGCTTGCCCGCCGC NGG 10 8 17 8 24 0 100 5 10
    240613161754_8c9y CGCAGGCGGCGGGCAAGCAGAGCCTCTGGATG(+AGGACA)GATCCTTCCTCAGAAGCTTCGATAATTTGCA NGG 10 8 17 8 24 0 100 5 10
    240613161753_pptw CCAGGCCTCCTGCATCCTGCCCAGACAGGCAG(+AGGACA)GTACCTGTCTCCCAGTCACAGCTGGGTGTGC NGG 10 8 17 8 24 0 100 5 10
    240613161752_hiff CACCTGCACACCCAGCTGTGACTGGGAGACAG(+AGGACA)GTACCTGCCTGTCTGGGCAGGATGCAGGAGG NGG 10 8 17 8 24 0 100 5 10
    240613161750_crs2 CAGGCCCAAGACGTCCCAGTGTGCCCCAAATG(+AGGACA)GATCCTACTTATTTCAAGTCAGCTGAGCCCA NGG 10 8 17 8 24 0 100 5 10
    240613161749_aul9 TGCCCTGGGCTCAGCTGACTTGAAATAAGTAG(+AGGACA)GATCCATTTGGGGCACACTGGGACGTCTTGG NGG 10 8 17 8 24 0 100 5 10
    240613161747_9kgy GCAACTATTTCTAGGGCAGAGCAAAGGCCTGG(+AGGACA)GATCCGGGATGAGCTTCAGGTTCTCCAGCTC NGG 10 8 17 8 24 0 100 5 10