Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613161816_6rb9 TGCTGCACACTGGGATTGGAGTTTCCTGTAAG(+AGGACA)GATCCCACCAGTCAGTGTGGCGTGCCATGAC NGG 10 8 17 8 24 0 100 5 10
    240613161815_xv9g TTGTTTGGTTCTTGTAGGCTGGTTGGTTTCAG(+AGGACA)GTACCTGAGACAATAGCACCAAATTCAACAG NGG 10 8 17 8 24 0 100 5 10
    240613161813_s3qg TTTCTCTGTTGAATTTGGTGCTATTGTCTCAG(+AGGACA)GTACCTGAAACCAACCAGCCTACAAGAACCA NGG 10 8 17 8 24 0 100 5 10
    240613161812_mozs TCTAGCCCACAGCTCCTTGCCTGTTGGACCTG(+AGGACA)GATCCTTTCAGGCTTGTACCCCAGACTGCTC NGG 10 8 17 8 24 0 100 5 10
    240613161810_a4v5 AGGTGGAGCAGTCTGGGGTACAAGCCTGAAAG(+AGGACA)GATCCAGGTCCAACAGGCAAGGAGCTGTGGG NGG 10 8 17 8 24 0 100 5 10
    240613161808_dmne GGGCCGGGGGCACACCACTCAGAGAAAGCAGG(+AGGACA)GTACCTGTCTGGCATGTTCTGGATAGGTCTG NGG 10 8 17 8 24 0 100 5 10
    240613161807_47xr GGGCTCAGACCTATCCAGAACATGCCAGACAG(+AGGACA)GTACCCTGCTTTCTCTGAGTGGTGTGCCCCC NGG 10 8 17 8 24 0 100 5 10
    240613161805_jsoa ATCCAGACAGTCCTGAGAGCGGAAGTGCTCGG(+AGGACA)GATCCCTGCTACCTCAGGACCTGGATTAAAA NGG 10 8 17 8 24 0 100 5 10
    240613161804_gs42 CAGCCTTTTAATCCAGGTCCTGAGGTAGCAGG(+AGGACA)GATCCCGAGCACTTCCGCTCTCAGGACTGTC NGG 10 8 17 8 24 0 100 5 10
    240613161802_cvrj TGTGTTTCTCTGCTCTGCTCCGAGGCTAATTG(+AGGACA)GTACCATGTTACCCCCTTGCCTTGTTACTCT NGG 10 8 17 8 24 0 100 5 10