Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613161916_joqj TGGTAAGGATGTGGTTCTGTGTAGAAAACGTG(+AGGACA)GTACCAAGTGAGAACAGCATTTCATGTCCTG NGG 10 8 17 8 24 0 100 5 10
    240613161914_canh TATGCAGAATCCTTGGGAAGCTAGAATATGGG(+AGGACA)GTACCATCCTCATAGCCCCAGAGAGTGGGAG NGG 10 8 17 8 24 0 100 5 10
    240613161912_76qj GGCTTCTCCCACTCTCTGGGGCTATGAGGATG(+AGGACA)GTACCCCATATTCTAGCTTCCCAAGGATTCT NGG 10 8 17 8 24 0 100 5 10
    240613161911_voj5 AGTGAGCCATCTGTGAGATCTCCATAGTCTAG(+AGGACA)GATCCACTGGTCCCAGGGGGACTGAGTGACT NGG 10 8 17 8 24 0 100 5 10
    240613161909_gjok CTATCAGTCACTCAGTCCCCCTGGGACCAGTG(+AGGACA)GATCCTAGACTATGGAGATCTCACAGATGGC NGG 10 8 17 8 24 0 100 5 10
    240613161908_b4zm GAGGGTCCAGCCACCACTGTCTTCTTTCTCTG(+AGGACA)GATCCAGAACAAGGAAATCTAGATGAGCCGG NGG 10 8 17 8 24 0 100 5 10
    240613161907_qlxq GCCACCCGGCTCATCTAGATTTCCTTGTTCTG(+AGGACA)GATCCAGAGAAAGAAGACAGTGGTGGCTGGA NGG 10 8 17 8 24 0 100 5 10
    240613161906_v1x5 GTGTGCAGTTCAATCCCGAGGACTGGAATTTG(+AGGACA)GATCCCACCACACACATAACAAGCCACATAT NGG 10 8 17 8 24 0 100 5 10
    240613161904_4xm0 ACAGAATATGTGGCTTGTTATGTGTGTGGTGG(+AGGACA)GATCCAAATTCCAGTCCTCGGGATTGAACTG NGG 10 8 17 8 24 0 100 5 10
    240613161902_b6m5 GCCCCAAGGTCACGTGTTAGGATACTATATGG(+AGGACA)GTACCATACATTTCAAAGTTGCTCATGGAAA NGG 10 8 17 8 24 0 100 5 10