Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613162005_9vhp CCTGGTGTATGGGGCCGGGGATGGGGGTCCTG(+AGGACA)GATCCCTGCACTCCCCATGCTTAAGGCCCTG NGG 10 8 17 8 24 0 100 5 10
    240613162003_7zp2 CCACCTATAGTTTGTTGAATCTGCAGATTTGG(+AGGACA)GATCCATGAGTGGGCAGGGCTCAATGTATTT NGG 10 8 17 8 24 0 100 5 10
    240613162002_rkoq AGGGGAAATACATTGAGCCCTGCCCACTCATG(+AGGACA)GATCCCAAATCTGCAGATTCAACAAACTATA NGG 10 8 17 8 24 0 100 5 10
    240613162000_b5i3 ATCAACTAGATTCCTCAGAGAGCTGGCACCAG(+AGGACA)GTACCTAAGACCGATGATACAGATGCGTGTG NGG 10 8 17 8 24 0 100 5 10
    240613161959_uo37 ACCCCCACACGCATCTGTATCATCGGTCTTAG(+AGGACA)GTACCTGGTGCCAGCTCTCTGAGGAATCTAG NGG 10 8 17 8 24 0 100 5 10
    240613161955_3d0b ACAGTCCGTGAGACTGGCTATGGCTAGCCTTG(+AGGACA)GTACCAGTAACAAAACGGAACATAACGGTTA NGG 10 8 17 8 24 0 100 5 10
    240613161954_ohub TTGTTTAACCGTTATGTTCCGTTTTGTTACTG(+AGGACA)GTACCAAGGCTAGCCATAGCCAGTCTCACGG NGG 10 8 17 8 24 0 100 5 10
    240613161952_5xmu GTTTTTAGGCTCAGCTACTCCTGGAAATACAG(+AGGACA)GATCCTGGGTATACAGTTAAATAGCTTCTCA NGG 10 8 17 8 24 0 100 5 10
    240613161950_k9x6 GGCGGTGAGAAGCTATTTAACTGTATACCCAG(+AGGACA)GATCCTGTATTTCCAGGAGTAGCTGAGCCTA NGG 10 8 17 8 24 0 100 5 10
    240613161948_whu2 CCTGGTGTATGGGGCCGGGGATGGGGGTCCTG(+AGGACA)GATCCCTGCACTCCCCATGCTCCAAGCCCTG NGG 10 8 17 8 24 0 100 5 10