Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613162036_5kol GCAGTGCCTGTCTTGCGTGGGGCACCGACCTG(+AGGACA)GATCCCTACTGGTCAAGTCCCCGCGGTCAGC NGG 10 8 17 8 24 0 100 5 10
    240613162034_grsb TGTGACCAGTCTCATTTCTGTTGCTCAGTGTG(+AGGACA)GTACCTCAGAACTGAAAATGGAGTTCCCTGA NGG 10 8 17 8 24 0 100 5 10
    240613162033_0yce CTGTCTCAGGGAACTCCATTTTCAGTTCTGAG(+AGGACA)GTACCACACTGAGCAACAGAAATGAGACTGG NGG 10 8 17 8 24 0 100 5 10
    240613162031_prrm CGGCTACCCCGAGCCTTACCCCTGTGCTCTGG(+AGGACA)GATCCAGATTTCCATATCTGTTAACTAAAGG NGG 10 8 17 8 24 0 100 5 10
    240613162030_f7p2 ACAGCCCTTTAGTTAACAGATATGGAAATCTG(+AGGACA)GATCCCAGAGCACAGGGGTAAGGCTCGGGGT NGG 10 8 17 8 24 0 100 5 10
    240613162028_0ag7 TGACTTTCTCATGGGACTGTCACTTGGGGTGG(+AGGACA)GATCCTATCCTGAGTCAAGAAGGGGTCGATC NGG 10 8 17 8 24 0 100 5 10
    240613162027_2350 AGAAGGATCGACCCCTTCTTGACTCAGGATAG(+AGGACA)GATCCCACCCCAAGTGACAGTCCCATGAGAA NGG 10 8 17 8 24 0 100 5 10
    240613162025_7ofi CTGAAACTTATGAACTGATCAGGAAGCTGTAG(+AGGACA)GTACCATGTTCTAGATTTGCTCATGAAGACA NGG 10 8 17 8 24 0 100 5 10
    240613162024_s8xj TCTTGTGTCTTCATGAGCAAATCTAGAACATG(+AGGACA)GTACCTACAGCTTCCTGATCAGTTCATAAGT NGG 10 8 17 8 24 0 100 5 10
    240613162022_injq CTTGTAAAAGCCAAGGACAGGATCTCCCTAAG(+AGGACA)GATCCAAGTACCCATTGTTTGAAGTATACTT NGG 10 8 17 8 24 0 100 5 10