Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613161930_xzyf TCAGCTGCAGTGAAATGCTGGAGATAGTGCAG(+AGGACA)GTACCTGCACTTGACAGGGCCTGGAGCATGG NGG 10 8 17 8 24 0 100 5 10
    240613161928_qbx7 AGGGGAAATACATTGAGCCCTGCCCACCCATG(+AGGACA)GATCCCGAATCTGCAGATTCAACAAACTATA NGG 10 8 17 8 24 0 100 5 10
    240613161927_154p CCACCTATAGTTTGTTGAATCTGCAGATTCGG(+AGGACA)GATCCATGGGTGGGCAGGGCTCAATGTATTT NGG 10 8 17 8 24 0 100 5 10
    240613161925_pgtl TCCTGGAGTGTGAGTTGTGTTGAACCTCAGTG(+AGGACA)GATCCAACAACTTAAAAGCTTTACCACACTG NGG 10 8 17 8 24 0 100 5 10
    240613161924_1fxr ATCAGCAGTGTGGTAAAGCTTTTAAGTTGTTG(+AGGACA)GATCCACTGAGGTTCAACACAACTCACACTC NGG 10 8 17 8 24 0 100 5 10
    240613161923_ftai CCTGGTGTATGGGGTCGGGGATAGGGGTCTTG(+AGGACA)GATCCCTGCACTCCCCATGCTCCAGACCCTG NGG 10 8 17 8 24 0 100 5 10
    240613161921_ixlq CTTGACAGGGTCTGGAGCATGGGGAGTGCAGG(+AGGACA)GATCCAAGACCCCTATCCCCGACCCCATACA NGG 10 8 17 8 24 0 100 5 10
    240613161920_3xk5 CCTGGTGTATGGGGTCGGGGATAGGGGTCTTG(+AGGACA)GATCCCTGCACTCCCCATGCTCCAGACCCTG NGG 10 8 17 8 24 0 100 5 10
    240613161918_nq25 CTTGACAGGGTCTGGAGCATGGGGAGTGCAGG(+AGGACA)GATCCAAGACCCCTATCCCCGACCCCATACA NGG 10 8 17 8 24 0 100 5 10
    240613161917_hpeg GACTCCAGGACATGAAATGCTGTTCTCACTTG(+AGGACA)GTACCACGTTTTCTACACAGAACCACATCCT NGG 10 8 17 8 24 0 100 5 10