Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613162142_4wcd GCAGCTGGTTCTCTCAGCATGTGGGTCTCCAG(+AGGACA)GTACCAAAAGGCATGAGGCTTGGCAGCAAGC NGG 10 8 17 8 24 0 100 5 10
    240613162141_55e7 AAACCGCTTGCTGCCAAGCCTCATGCCTTTTG(+AGGACA)GTACCTGGAGACCCACATGCTGAGAGAACCA NGG 10 8 17 8 24 0 100 5 10
    240613162139_ozey TTACTTGATACACCGTGGAATGAAAATGTAAG(+AGGACA)GTACCTTTCAGTATGTGCTCTGAGCTTTAAT NGG 10 8 17 8 24 0 100 5 10
    240613162138_apq6 AAAAAATTAAAGCTCAGAGCACATACTGAAAG(+AGGACA)GTACCTTACATTTTCATTCCACGGTGTATCA NGG 10 8 17 8 24 0 100 5 10
    240613162136_ek0c CAGCAGTGAAACATTTAAATGCATATCAATGG(+AGGACA)GTACCATGTCTAAAAATTACTATAGTACCTA NGG 10 8 17 8 24 0 100 5 10
    240613162135_mubh CTAAATAGGTACTATAGTAATTTTTAGACATG(+AGGACA)GTACCCATTGATATGCATTTAAATGTTTCAC NGG 10 8 17 8 24 0 100 5 10
    240613162134_nfxj GATTTGCTTCAATGATCTCAGTATTATTGCAG(+AGGACA)GTACCAATCTACCCAAGAGGAACCAATGCTG NGG 10 8 17 8 24 0 100 5 10
    240613162132_gzls AATGACAGCATTGGTTCCTCTTGGGTAGATTG(+AGGACA)GTACCTGCAATAATACTGAGATCATTGAAGC NGG 10 8 17 8 24 0 100 5 10
    240613162131_g7ui TTCATTTTTCATTAACTAAGTTGCTCTTGTAG(+AGGACA)GTACCATGTAAGATACCACTCTTTCTCTGTG NGG 10 8 17 8 24 0 100 5 10
    240613162130_96c0 ATCATCACAGAGAAAGAGTGGTATCTTACATG(+AGGACA)GTACCTACAAGAGCAACTTAGTTAATGAAAA NGG 10 8 17 8 24 0 100 5 10