Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613161604_qflr TTGCTTGCTGCTTCCTGGTGGCCCTTGGTTAG(+AGGACA)GATCCCTCTCTTTTCCTTCTGGAGCTCAATG NGG 10 8 17 8 24 0 100 5 10
    240613161602_5hi5 CAGGAGTCCCTGAGGTTCCCAGCTGCTGTCAG(+AGGACA)GATCCTGTGAGTTGGGGCAAGCAGGATGCTG NGG 10 8 17 8 24 0 100 5 10
    240613161601_yhnm GGTGGCAGCATCCTGCTTGCCCCAACTCACAG(+AGGACA)GATCCTGACAGCAGCTGGGAACCTCAGGGAC NGG 10 8 17 8 24 0 100 5 10
    240613161559_kck5 CTGTCACATGCAGCAGACAGGCAGCATTAAGG(+AGGACA)GTACCCTAGCTCTGTTAGGGCAGAGCCTGGG NGG 10 8 17 8 24 0 100 5 10
    240613161556_6szd ACATTCCCAGGCTCTGCCCTAACAGAGCTAGG(+AGGACA)GTACCCTTAATGCTGCCTGTCTGCTGCATGT NGG 10 8 17 8 24 0 100 5 10
    240613161555_ldio GGCGGCACTCGCCCCTCCTGGGGATACAGCAG(+AGGACA)GATCCGGCCTCAAGAGGCTTCAGGTGCTTGA NGG 10 8 17 8 24 0 100 5 10
    240613161553_i50y GAGTGTCAAGCACCTGAAGCCTCTTGAGGCCG(+AGGACA)GATCCTGCTGTATCCCCAGGAGGGGCGAGTG NGG 10 8 17 8 24 0 100 5 10
    240613161550_ptv0 GGTGTAGCCCTACCTACAGTGTGACTAGGCCG(+AGGACA)GTACCGATCCTGCCTGGTTCTAACCAGAAAA NGG 10 8 17 8 24 0 100 5 10
    240613161549_2zw8 GTCTGTTTTCTGGTTAGAACCAGGCAGGATCG(+AGGACA)GTACCGGCCTAGTCACACTGTAGGTAGGGCT NGG 10 8 17 8 24 0 100 5 10
    240613161547_y38q AACCCCTTTCAACAGCCCTCCCTCTTCAGTAG(+AGGACA)GTACCCGCCCCTAGTGATGATGGGCAGACAG NGG 10 8 17 8 24 0 100 5 10