Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613161453_yhsb ATTGTAAATTGCTTAAAAAACATCCTGTGGTG(+AGGACA)GATCCATGAAACAGCCAGGAAAGGCTCCCTC NGG 10 8 17 8 24 0 100 5 10
    240613161451_k8v5 TAAGATGGGGAACACATGGGGGAGGGGCTGAG(+AGGACA)GATCCCTTTACCAAGGGCCTGAGGTCATACA NGG 10 8 17 8 24 0 100 5 10
    240613161450_33ma TTGAATGTATGACCTCAGGCCCTTGGTAAAGG(+AGGACA)GATCCTCAGCCCCTCCCCCATGTGTTCCCCA NGG 10 8 17 8 24 0 100 5 10
    240613161447_lc2x CCCTGGTTGGGGTTCACCCGAGATGCCCAGGG(+AGGACA)GTACCGCCTCCAGCCTCAGGCCTGGAGCATT NGG 10 8 17 8 24 0 100 5 10
    240613161446_dr9q GATGGAATGCTCCAGGCCTGAGGCTGGAGGCG(+AGGACA)GTACCCCTGGGCATCTCGGGTGAACCCCAAC NGG 10 8 17 8 24 0 100 5 10
    240613161443_85qd ACAGAAGTCACTGTCTTGCTGTCTAACCCAAG(+AGGACA)GATCCTATGTAGGGCCTCTCCAGAGAGACAA NGG 10 8 17 8 24 0 100 5 10
    240613161442_iza1 ATGCCTTGTCTCTCTGGAGAGGCCCTACATAG(+AGGACA)GATCCTTGGGTTAGACAGCAAGACAGTGACT NGG 10 8 17 8 24 0 100 5 10
    240613161441_d85o CCTCTCCTGAGTCAGTTCTCTCTACCGTGTAG(+AGGACA)GTACCAGGGATCTAACTCGTGGCAGTGTGGA NGG 10 8 17 8 24 0 100 5 10
    240613161438_ne09 CGCTGTCCACACTGCCACGAGTTAGATCCCTG(+AGGACA)GTACCTACACGGTAGAGAGAACTGACTCAGG NGG 10 8 17 8 24 0 100 5 10
    240613161437_4vy8 GGACAAAAGGTTCCAAGTCGTGTGAACAGAAG(+AGGACA)GATCCAGCTGCCAACTGGCATCCCAGTGCCA NGG 10 8 17 8 24 0 100 5 10