Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604235148_smsb CAACCTGTGAGGATTCTACCTGCCTCTACGGG(+ACTCAC)GTACCTGTTGCTTAAAGAAAAAACAGGGGGC NGG 10 8 17 8 24 0 100 5 10
    240604235146_e486 CATTTTCTCAGCTCCCGGGGATGGGAATGGAG(+ACTCAC)GATCCCAGAACACTCAGCCCAGCCCAGCCCC NGG 10 8 17 8 24 0 100 5 10
    240604235145_uq7k TGAAAGGGGCTGGGCTGGGCTGAGTGTTCTGG(+ACTCAC)GATCCTCCATTCCCATCCCCGGGAGCTGAGA NGG 10 8 17 8 24 0 100 5 10
    240604235143_qf5s AAGGGGCACTCTGTGAGCTGCTCTGAGCCATG(+ACTCAC)GATCCAACCCCAGAGCTGGTAAGATACCAGT NGG 10 8 17 8 24 0 100 5 10
    240604235141_9eed GGGAGACTGGTATCTTACCAGCTCTGGGGTTG(+ACTCAC)GATCCATGGCTCAGAGCAGCTCACAGAGTGC NGG 10 8 17 8 24 0 100 5 10
    240604235140_x774 AGATTTTTAGGGATGTATGGTATTGCTGCCAG(+ACTCAC)GTACCGTGGGGGCAGCCGGGATGGGAGGACC NGG 10 8 17 8 24 0 100 5 10
    240604235137_93we ACACTGGTCCTCCCATCCCGGCTGCCCCCACG(+ACTCAC)GTACCTGGCAGCAATACCATACATCCCTAAA NGG 10 8 17 8 24 0 100 5 10
    240604235136_o06f ATTGGCCACTGGAGAACGTTACTGTCACTGAG(+ACTCAC)GATCCTCTAAGCACAGGGAGAACCCTAGATC NGG 10 8 17 8 24 0 100 5 10
    240604235134_9qsw CTCCAGATCTAGGGTTCTCCCTGTGCTTAGAG(+ACTCAC)GATCCTCAGTGACAGTAACGTTCTCCAGTGG NGG 10 8 17 8 24 0 100 5 10
    240604235133_d7xg CAGCCTGCCCTGTCTGTCACTGAGGGCTCTGG(+ACTCAC)GATCCTAGTGGAGACAGGGACCGCTTAGAAG NGG 10 8 17 8 24 0 100 5 10