Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604235101_b9cn GAGTATGGTGTGGTATATATTTGATATTCTAG(+ACTCAC)GATCCAAAACAGGCCCTTGTACCCAGAAGGG NGG 10 8 17 8 24 0 100 5 10
    240604235059_y4oy GCCCCCACAACCTAGCCCAAGAACCAGCCTGG(+ACTCAC)GTACCCATGGATTATATTGGGAGGGGCAGCA NGG 10 8 17 8 24 0 100 5 10
    240604235056_n1me CTCTCTGCTGCCCCTCCCAATATAATCCATGG(+ACTCAC)GTACCCAGGCTGGTTCTTGGGCTAGGTTGTG NGG 10 8 17 8 24 0 100 5 10
    240604235054_y4mx CAGGGAATGAGATTGGCCCTTTCTCTGCCAGG(+ACTCAC)GTACCACAGGGACACATGCCGGGTAGCCTGA NGG 10 8 17 8 24 0 100 5 10
    240604235052_dur2 CATACTCAGGCTACCCGGCATGTGTCCCTGTG(+ACTCAC)GTACCCTGGCAGAGAAAGGGCCAATCTCATT NGG 10 8 17 8 24 0 100 5 10
    240604235050_sdf7 TTGGTGAGGGTCCTGAGGTGCTCTGGCCACAG(+ACTCAC)GTACCATCCATAGCTCCAACTCATGTGACCA NGG 10 8 17 8 24 0 100 5 10
    240604235049_vpas CCCTTTGGTCACATGAGTTGGAGCTATGGATG(+ACTCAC)GTACCTGTGGCCAGAGCACCTCAGGACCCTC NGG 10 8 17 8 24 0 100 5 10
    240604235046_x8k3 ATTAAAAGAGCTTCCTGACAGGGGCCCCTTGG(+ACTCAC)GATCCACCCAAGTGAGACTGCAGAGCCCAAC NGG 10 8 17 8 24 0 100 5 10
    240604235044_rpen TCCAAGTTGGGCTCTGCAGTCTCACTTGGGTG(+ACTCAC)GATCCCAAGGGGCCCCTGTCAGGAAGCTCTT NGG 10 8 17 8 24 0 100 5 10
    240604235042_58mx GCCCCCACAACCTAGCCCAAGAACCAGCCTGG(+ACTCAC)GTACCCATGGATTATATTGGGAGGGGCAGCA NGG 10 8 17 8 24 0 100 5 10