Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604235022_gkpi TAGTTGCCTGTCTCGGGAGGTTTGGTGTTGTG(+ACTCAC)GTACCCAATGAATGTTTGCCCAAAGAGCAGG NGG 10 8 17 8 24 0 100 5 10
    240604235020_tt74 ATATCGGCTGAATACCGGCTGAACTAAAACTG(+ACTCAC)GTACCCGCTGCCTTCAGTCCAGTGGGTCACA NGG 10 8 17 8 24 0 100 5 10
    240604235019_0qmf CTGCCTGTGACCCACTGGACTGAAGGCAGCGG(+ACTCAC)GTACCAGTTTTAGTTCAGCCGGTATTCAGCC NGG 10 8 17 8 24 0 100 5 10
    240604235017_r1n5 AAAGACTCCCACCCCTGGCAGGAAGCAGGAAG(+ACTCAC)GATCCGCCAGGCCTGGAGCATTCCTTCCTCC NGG 10 8 17 8 24 0 100 5 10
    240604235016_ckkj ACTGTGGAGGAAGGAATGCTCCAGGCCTGGCG(+ACTCAC)GATCCTTCCTGCTTCCTGCCAGGGGTGGGAG NGG 10 8 17 8 24 0 100 5 10
    240604235013_1g4p AACAATATAAAAAAGTACTCCCATAGTCCCAG(+ACTCAC)GTACCTCCATCAAAAAAAAAAAAGAACTGGG NGG 10 8 17 8 24 0 100 5 10
    240604235012_mzib TTGTCCCCAGTTCTTTTTTTTTTTTGATGGAG(+ACTCAC)GTACCTGGGACTATGGGAGTACTTTTTTATA NGG 10 8 17 8 24 0 100 5 10
    240604235009_d6ky GGGAAAATCTAAAGGAGACCCCACGTGCCCAG(+ACTCAC)GATCCGGGAACCAACCTTCCACACCACTCCA NGG 10 8 17 8 24 0 100 5 10
    240604235007_d9y3 GGGGCTGGAGTGGTGTGGAAGGTTGGTTCCCG(+ACTCAC)GATCCTGGGCACGTGGGGTCTCCTTTAGATT NGG 10 8 17 8 24 0 100 5 10
    240604235005_oj1r GTCTCTTAAGGGGCAGCCCTAGGGTAGGATAG(+ACTCAC)GATCCTACCTCTCGCCAGCATCCCCCATGGA NGG 10 8 17 8 24 0 100 5 10