Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604234930_17d3 CTCTGAAGCTATAGCATTGGTTTCCCCAGTGG(+ACTCAC)GATCCCCCAGGGAGCTTGTACGGTTGGCTTG NGG 10 8 17 8 24 0 100 5 10
    240604234929_1eaz ATGGAACAGAGATCGGTATCCTATTGGGAAAG(+ACTCAC)GTACCCGGTCTAACCCTTCCAATCTATCCTA NGG 10 8 17 8 24 0 100 5 10
    240604234926_nstd CATTTTAGGATAGATTGGAAGGGTTAGACCGG(+ACTCAC)GTACCTTTCCCAATAGGATACCGATCTCTGT NGG 10 8 17 8 24 0 100 5 10
    240604234925_4cjl GTCCCCCACATCCCCTTCTGTACCCCTTCCTG(+ACTCAC)GATCCCCACTGCTGGGGCCAGGAAAGCCCTC NGG 10 8 17 8 24 0 100 5 10
    240604234923_n0qk AACCCGAGGGCTTTCCTGGCCCCAGCAGTGGG(+ACTCAC)GATCCAGGAAGGGGTACAGAAGGGGATGTGG NGG 10 8 17 8 24 0 100 5 10
    240604234921_lgaj GTATTTAGTCTACATCCATTCCAATTTCCAGG(+ACTCAC)GATCCATCGATCCATTGACCCAGGGATGAGG NGG 10 8 17 8 24 0 100 5 10
    240604234919_9b9m CAGTCCCTCATCCCTGGGTCAATGGATCGATG(+ACTCAC)GATCCCTGGAAATTGGAATGGATGTAGACTA NGG 10 8 17 8 24 0 100 5 10
    240604234918_l61w AGTTCCCAAATCTCCAGTCTAAATCCTTTTTG(+ACTCAC)GATCCCACAGGACCCCACCACACTCCTTTCC NGG 10 8 17 8 24 0 100 5 10
    240604234917_ph7y GGGGGGGAAAGGAGTGTGGTGGGGTCCTGTGG(+ACTCAC)GATCCAAAAAGGATTTAGACTGGAGATTTGG NGG 10 8 17 8 24 0 100 5 10
    240604234915_1un9 TGCTGAGACATGCTGGGCATGGCAGCTGTGAG(+ACTCAC)GTACCTCCCACATCTGCTTTCTGGCTGTGCT NGG 10 8 17 8 24 0 100 5 10