Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604234913_84fj AAGTCAGCACAGCCAGAAAGCAGATGTGGGAG(+ACTCAC)GTACCTCACAGCTGCCATGCCCAGCATGTCT NGG 10 8 17 8 24 0 100 5 10
    240604234911_qfxf CTCTTGTAGAAGCCAGTCAGGAACTGAGCCTG(+ACTCAC)GATCCTGTCCACATAAGCCTGCCCAGAGCAG NGG 10 8 17 8 24 0 100 5 10
    240604234910_efd5 TAGTGCTGCTCTGGGCAGGCTTATGTGGACAG(+ACTCAC)GATCCAGGCTCAGTTCCTGACTGGCTTCTAC NGG 10 8 17 8 24 0 100 5 10
    240604234908_qtm9 GCGCCCCCTGGCGGGACCCTTGACCCCCAAAG(+ACTCAC)GATCCACCAGAGCAGTTGCTGTGCTCTCTAG NGG 10 8 17 8 24 0 100 5 10
    240604234907_azb3 ATATTCTAGAGAGCACAGCAACTGCTCTGGTG(+ACTCAC)GATCCTTTGGGGGTCAAGGGTCCCGCCAGGG NGG 10 8 17 8 24 0 100 5 10
    240604234903_7reo GCTCCATTATTCCCCACACAAGACTCCCCTGG(+ACTCAC)GTACCACGGAGAACTCCTCTAAAACCTCTTA NGG 10 8 17 8 24 0 100 5 10
    240604234901_vvr1 GCACCTAAGAGGTTTTAGAGGAGTTCTCCGTG(+ACTCAC)GTACCCAGGGGAGTCTTGTGTGGGGAATAAT NGG 10 8 17 8 24 0 100 5 10
    240604234859_xbpw GCCGCCTGGAAGCGCTCAGTGCCACGGCTGGG(+ACTCAC)GATCCTAGATCCTGCTGCATGGACCCTATAA NGG 10 8 17 8 24 0 100 5 10
    240604234858_ctlh ACAATTTATAGGGTCCATGCAGCAGGATCTAG(+ACTCAC)GATCCCCAGCCGTGGCACTGAGCGCTTCCAG NGG 10 8 17 8 24 0 100 5 10
    240604234855_mvaa TCAATAATTCTGTGCCTCGCATTCCTTCCTGG(+ACTCAC)GATCCCAGCCCTGACTTTCCAGAAGAGTCAA NGG 10 8 17 8 24 0 100 5 10