Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604234854_1ncy AACACTTGACTCTTCTGGAAAGTCAGGGCTGG(+ACTCAC)GATCCCAGGAAGGAATGCGAGGCACAGAATT NGG 10 8 17 8 24 0 100 5 10
    240604234853_jppn AGGGACGGGAGCAAAGAGGCTGCCGTAACTAG(+ACTCAC)GTACCTAGTGGAGGGAAATGGTCACCAAGCC NGG 10 8 17 8 24 0 100 5 10
    240604234851_rl7o CATCAGGCTTGGTGACCATTTCCCTCCACTAG(+ACTCAC)GTACCTAGTTACGGCAGCCTCTTTGCTCCCG NGG 10 8 17 8 24 0 100 5 10
    240604234850_5exx AAAGCTGTCACATGAAAGCCATCCCAGAAATG(+ACTCAC)GTACCTTTTTTTTTTTTTTTTTCCTAAAGAC NGG 10 8 17 8 24 0 100 5 10
    240604234849_q3jl ATTCAGTCTTTAGGAAAAAAAAAAAAAAAAAG(+ACTCAC)GTACCATTTCTGGGATGGCTTTCATGTGACA NGG 10 8 17 8 24 0 100 5 10
    240604234847_59wn GAAGAGCAAACCCATAGTCTTGCCTTCTCCTG(+ACTCAC)GATCCAGAGCTTTCGGAGAACCTAACTGTGA NGG 10 8 17 8 24 0 100 5 10
    240604234846_go0i TTTGGTCACAGTTAGGTTCTCCGAAAGCTCTG(+ACTCAC)GATCCAGGAGAAGGCAAGACTATGGGTTTGC NGG 10 8 17 8 24 0 100 5 10
    240604234845_59qu GGGCACTGGCGCTGGCCTGCTGCCTGCAGCTG(+ACTCAC)GTACCCTGCCCAAGCTGTCTTCTCTGCAGCC NGG 10 8 17 8 24 0 100 5 10
    240604234843_x6fj CAGGCGGCTGCAGAGAAGACAGCTTGGGCAGG(+ACTCAC)GTACCAGCTGCAGGCAGCAGGCCAGCGCCAG NGG 10 8 17 8 24 0 100 5 10
    240604234841_gvk5 TCTTACCCTCAATGCCAAACAGCTGGCACTGG(+ACTCAC)GTACCTAGGTTCAGGCCAGAAGCCAGCCAGG NGG 10 8 17 8 24 0 100 5 10