Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604234824_0p9p GGAGATGTCCAGGCCAGTGCTACACACAGCAG(+ACTCAC)GATCCCACCTACACGGACTCCAAACAGAACT NGG 10 8 17 8 24 0 100 5 10
    240604234822_u6ss TGTAGGGGGTTGAGAGGAAGGGAATGAGCCAG(+ACTCAC)GTACCCTGCATTCCAAGGGAGTTTCAGTGTG NGG 10 8 17 8 24 0 100 5 10
    240604234820_u0h2 CTTTGCACACTGAAACTCCCTTGGAATGCAGG(+ACTCAC)GTACCTGGCTCATTCCCTTCCTCTCAACCCC NGG 10 8 17 8 24 0 100 5 10
    240604234818_f3bs GAAGGTTTAGAGAATCCGATGGGCGCTGCCTG(+ACTCAC)GATCCCGGCATAGCCCCAGACACTTGGTTCC NGG 10 8 17 8 24 0 100 5 10
    240604234816_3g6r ATAAAGGAACCAAGTGTCTGGGGCTATGCCGG(+ACTCAC)GATCCAGGCAGCGCCCATCGGATTCTCTAAA NGG 10 8 17 8 24 0 100 5 10
    240604234815_98vf GCTTTAGATAAGTCCCTGACACCAAGCTTCAG(+ACTCAC)GATCCAAGTGTTAACAGGAAAGAGCTGAAAT NGG 10 8 17 8 24 0 100 5 10
    240604234814_sha6 CATTTATTTCAGCTCTTTCCTGTTAACACTTG(+ACTCAC)GATCCTGAAGCTTGGTGTCAGGGACTTATCT NGG 10 8 17 8 24 0 100 5 10
    240604234813_wkpw ATCTCCAAGTCTTCTTCCAGAGGATGCATTTG(+ACTCAC)GATCCCAGAGGAGTGTTTTCTGGAAGACAAT NGG 10 8 17 8 24 0 100 5 10
    240604234811_jnuy GAAAGATTGTCTTCCAGAAAACACTCCTCTGG(+ACTCAC)GATCCAAATGCATCCTCTGGAAGAAGACTTG NGG 10 8 17 8 24 0 100 5 10
    240604234809_hc72 ACCTCATCTCCTAAGATAGATTTTTGTCTTGG(+ACTCAC)GTACCTTATCACTTGTATCTTACTATAAAAG NGG 10 8 17 8 24 0 100 5 10