Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604234735_1zvk TACTATGATGCAGTGGCACTTGTCCAGAACTG(+ACTCAC)GTACCACCCCATAGCTGGGAAACTTTAAATG NGG 10 8 17 8 24 0 100 5 10
    240604234733_npr7 GGGCGGGAGTAAGGAAATGCAGGAGTTGACCG(+ACTCAC)GATCCTTGTTCCTCACCTTGTACACTCACTT NGG 10 8 17 8 24 0 100 5 10
    240604234731_0zin AGTTTAAGTGAGTGTACAAGGTGAGGAACAAG(+ACTCAC)GATCCGGTCAACTCCTGCATTTCCTTACTCC NGG 10 8 17 8 24 0 100 5 10
    240604234730_e0tm CATGCTATATGTATACTTCTGTTTTCTGTTGG(+ACTCAC)GTACCTTGCAATGCATTCTATGCCTCATTAA NGG 10 8 17 8 24 0 100 5 10
    240604234728_1h1b TCCATTTAATGAGGCATAGAATGCATTGCAAG(+ACTCAC)GTACCCAACAGAAAACAGAAGTATACATATA NGG 10 8 17 8 24 0 100 5 10
    240604234726_0hcq CAAGAAAATATCATCCTGAATGAAGTATCCTG(+ACTCAC)GATCCAAAAGGACATGCATGGTGCTTACTCA NGG 10 8 17 8 24 0 100 5 10
    240604234725_1cyg ATCAGTGAGTAAGCACCATGCATGTCCTTTTG(+ACTCAC)GATCCAGGATACTTCATTCAGGATGATATTT NGG 10 8 17 8 24 0 100 5 10
    240604234723_99dj GGAATGATTGACCAGTGACTATCCCAATGTGG(+ACTCAC)GATCCAGGAGACTAGCATGGCTGTCCTCTGA NGG 10 8 17 8 24 0 100 5 10
    240604234721_7dhn GCCTCTCAGAGGACAGCCATGCTAGTCTCCTG(+ACTCAC)GATCCCACATTGGGATAGTCACTGGTCAATC NGG 10 8 17 8 24 0 100 5 10
    240604234720_0v2r GGAAGGAATGAATAGGATGTGGCTAGCAGGAG(+ACTCAC)GATCCTGCAAGCCAAAAGAGCAGCTTAGACA NGG 10 8 17 8 24 0 100 5 10