Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604234648_53qy AATGAGAACTGAAGACTTGGCCTGCCTTGCGG(+ACTCAC)GATCCACATACACATATTGTCCACTTATAAA NGG 10 8 17 8 24 0 100 5 10
    240604234647_7i4x GGTCCCAGCTACGTGGGCAGCTAAGGCAGGAG(+ACTCAC)GATCCCATTAGCCCATAAGCTCAGGGCTATA NGG 10 8 17 8 24 0 100 5 10
    240604234645_1l2a CCCAGTATAGCCCTGAGCTTATGGGCTAATGG(+ACTCAC)GATCCTCCTGCCTTAGCTGCCCACGTAGCTG NGG 10 8 17 8 24 0 100 5 10
    240604234644_26cv AGCGCCAGCCCTAGGAACCCCAGAAAAGAGTG(+ACTCAC)GTACCTAAGGCTGAAATTGTTTTGCTGTTGC NGG 10 8 17 8 24 0 100 5 10
    240604234642_b57m CCCTGGCAACAGCAAAACAATTTCAGCCTTAG(+ACTCAC)GTACCACTCTTTTCTGGGGTTCCTAGGGCTG NGG 10 8 17 8 24 0 100 5 10
    240604234641_b573 TCTAAAATTTAAGCCCTTTAAATATCACAAAG(+ACTCAC)GTACCCTTGTTGGCCTTACAAAAGATAGCTA NGG 10 8 17 8 24 0 100 5 10
    240604234639_bsvx AAAGGTAGCTATCTTTTGTAAGGCCAACAAGG(+ACTCAC)GTACCTTTGTGATATTTAAAGGGCTTAAATT NGG 10 8 17 8 24 0 100 5 10
    240604234638_9paf TTTTTATAGCAAAGGGAATGTATCTTAACACG(+ACTCAC)GATCCTGTTTATCTATTGGGACTCCAAACGG NGG 10 8 17 8 24 0 100 5 10
    240604234636_qulf TTTGACCGTTTGGAGTCCCAATAGATAAACAG(+ACTCAC)GATCCGTGTTAAGATACATTCCCTTTGCTAT NGG 10 8 17 8 24 0 100 5 10
    240604234635_jje7 GTGGTCCCTAGGCAAAAGGAGGGGGATTCTGG(+ACTCAC)GATCCTAAAAGGCTTTATTTTTTGACTTAAA NGG 10 8 17 8 24 0 100 5 10