Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604234544_mj4z CTGGAAAGACAAGCGGTCAAAGCTGTGGCTTG(+ACTCAC)GATCCACACTATGGGCATTTTGGTGATTCTT NGG 10 8 17 8 24 0 100 5 10
    240604234543_vhwa AGGACAAGAATCACCAAAATGCCCATAGTGTG(+ACTCAC)GATCCAAGCCACAGCTTTGACCGCTTGTCTT NGG 10 8 17 8 24 0 100 5 10
    240604234542_n128 GGAGGTGACCCTTAAGGATCTAGATACTGATG(+ACTCAC)GTACCCAGCTGGCAAGTGGCACAGACCACTG NGG 10 8 17 8 24 0 100 5 10
    240604234540_x0if GAAGCCAGTGGTCTGTGCCACTTGCCAGCTGG(+ACTCAC)GTACCATCAGTATCTAGATCCTTAAGGGTCA NGG 10 8 17 8 24 0 100 5 10
    240604234538_m81t GCTCCCCCAATGCAGGGTAGGGTGCCAGGTGG(+ACTCAC)GATCCAAGACAGCCTTATGTCCTGTAAACAT NGG 10 8 17 8 24 0 100 5 10
    240604234537_qrj1 GGTTCATGTTTACAGGACATAAGGCTGTCTTG(+ACTCAC)GATCCCACCTGGCACCCTACCCTGCATTGGG NGG 10 8 17 8 24 0 100 5 10
    240604234535_nb45 TATGGATAAAATTGGTGCAAATTGGTCAAACG(+ACTCAC)GATCCAACAAACACTGATGTCCAAGCTGGCA NGG 10 8 17 8 24 0 100 5 10
    240604234534_nekw CAATATGCCAGCTTGGACATCAGTGTTTGTTG(+ACTCAC)GATCCGTTTGACCAATTTGCACCAATTTTAT NGG 10 8 17 8 24 0 100 5 10
    240604234533_2b6w ATTCCAAGGCCAAGTCCAGTCTATGAAACAAG(+ACTCAC)GTACCATACCCAGTCTGAGCATGGGATGCTA NGG 10 8 17 8 24 0 100 5 10
    240604234532_34rk AAAACTAGCATCCCATGCTCAGACTGGGTATG(+ACTCAC)GTACCTTGTTTCATAGACTGGACTTGGCCTT NGG 10 8 17 8 24 0 100 5 10