Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604234438_bsdt TGGAACTCACACCAGGCATAAACAGACAGAGG(+ACTCAC)GATCCGGAACGGCAAGACCTCGGAACACTAC NGG 10 8 17 8 24 0 100 5 10
    240604234437_nfuv TCTGAGTAGTGTTCCGAGGTCTTGCCGTTCCG(+ACTCAC)GATCCCTCTGTCTGTTTATGCCTGGTGTGAG NGG 10 8 17 8 24 0 100 5 10
    240604234435_wrsi GGAGGGACTCTCCTGGATTCAGACCTCCACAG(+ACTCAC)GTACCCTGTGACTACCAAAGTTCAAGGGGAA NGG 10 8 17 8 24 0 100 5 10
    240604234433_zhv9 AGCCATTCCCCTTGAACTTTGGTAGTCACAGG(+ACTCAC)GTACCTGTGGAGGTCTGAATCCAGGAGAGTC NGG 10 8 17 8 24 0 100 5 10
    240604234432_fp4q GGGGGGAGGGCTGCCCAGGATGGGAGGATGTG(+ACTCAC)GTACCACCATTATTGCTTCCTCCTAGGGCAA NGG 10 8 17 8 24 0 100 5 10
    240604234430_lj26 TCTCATTGCCCTAGGAGGAAGCAATAATGGTG(+ACTCAC)GTACCACATCCTCCCATCCTGGGCAGCCCTC NGG 10 8 17 8 24 0 100 5 10
    240604234428_xyl3 GCAGCTCACGTGTACACCCACCCTGGCGGGAG(+ACTCAC)GATCCGACTGAGGCCAGGGGTCTGGTCAGTC NGG 10 8 17 8 24 0 100 5 10
    240604234427_3qe7 CCGAGGACTGACCAGACCCCTGGCCTCAGTCG(+ACTCAC)GATCCTCCCGCCAGGGTGGGTGTACACGTGA NGG 10 8 17 8 24 0 100 5 10
    240604234425_2rym TTTCTCTATTGGAGACAATGGAGGCTGACAGG(+ACTCAC)GATCCCAGTGGGAGAGTCAGGCCTCAGGCCC NGG 10 8 17 8 24 0 100 5 10
    240604234423_xuxo ACTCTGGGCCTGAGGCCTGACTCTCCCACTGG(+ACTCAC)GATCCCTGTCAGCCTCCATTGTCTCCAATAG NGG 10 8 17 8 24 0 100 5 10