Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604234421_ren1 CATGAGCTCATCACCTCTTCATCCTATCCCAG(+ACTCAC)GTACCACGGCCCTTAAGGCAGGGCAGCCTTG NGG 10 8 17 8 24 0 100 5 10
    240604234419_20om CAGCTCAAGGCTGCCCTGCCTTAAGGGCCGTG(+ACTCAC)GTACCTGGGATAGGATGAAGAGGTGATGAGC NGG 10 8 17 8 24 0 100 5 10
    240604234418_bjnd GTTCCTTTATAAAAGGAAACCGGACTGCACTG(+ACTCAC)GATCCCAGGCAGCTTCCGGGCCCCAATCTGG NGG 10 8 17 8 24 0 100 5 10
    240604234416_sb8z ATGCCCCAGATTGGGGCCCGGAAGCTGCCTGG(+ACTCAC)GATCCAGTGCAGTCCGGTTTCCTTTTATAAA NGG 10 8 17 8 24 0 100 5 10
    240604234414_inio AACTGTGCCCTGTACCATCGTGGGGGCTGGTG(+ACTCAC)GTACCATGCCTGTGCCCACTCTAACCTCAAT NGG 10 8 17 8 24 0 100 5 10
    240604234413_syvp ACTCCATTGAGGTTAGAGTGGGCACAGGCATG(+ACTCAC)GTACCACCAGCCCCCACGATGGTACAGGGCA NGG 10 8 17 8 24 0 100 5 10
    240604234412_n8wf TTCAGGGGACCAAGATGTTGGGCATAAATCAG(+ACTCAC)GATCCTACATGGTGCTGCCCTGCTCATAACT NGG 10 8 17 8 24 0 100 5 10
    240604234410_m6zd TTCCCAGTTATGAGCAGGGCAGCACCATGTAG(+ACTCAC)GATCCTGATTTATGCCCAACATCTTGGTCCC NGG 10 8 17 8 24 0 100 5 10
    240604234409_kkdd AGACCTCATTATTTATGTGTTTGTATGTGTGG(+ACTCAC)GTACCCAGAGACCAGAAGAGGAAGAGTCTGA NGG 10 8 17 8 24 0 100 5 10
    240604234407_xtk4 GGAGGTCAGACTCTTCCTCTTCTGGTCTCTGG(+ACTCAC)GTACCCACACATACAAACACATAAATAATGA NGG 10 8 17 8 24 0 100 5 10