Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604234404_v4af GCTCACAAGCATCTGTAATTCCAGTTCCAGGG(+ACTCAC)GATCCAACACCCTCTTCTGGTTGGTCTCCAT NGG 10 8 17 8 24 0 100 5 10
    240604234403_wfvv TTCTCATGGAGACCAACCAGAAGAGGGTGTTG(+ACTCAC)GATCCCCTGGAACTGGAATTACAGATGCTTG NGG 10 8 17 8 24 0 100 5 10
    240604234401_oex1 AAGATGCCATGCACACTGTCAGAGGACAAAGG(+ACTCAC)GATCCTCAGTGTCACCCAGCTGCAGGTCGCT NGG 10 8 17 8 24 0 100 5 10
    240604234400_muo9 ACACCAGCGACCTGCAGCTGGGTGACACTGAG(+ACTCAC)GATCCCTTTGTCCTCTGACAGTGTGCATGGC NGG 10 8 17 8 24 0 100 5 10
    240604234359_p92t GAACTTGCTGCTAAGCCAGACAGCCTGAGTTG(+ACTCAC)GATCCCTGGGACTCATGGCCTGCTGCCGGCT NGG 10 8 17 8 24 0 100 5 10
    240604234356_69eh AAGACAGCCGGCAGCAGGCCATGAGTCCCAGG(+ACTCAC)GATCCAACTCAGGCTGTCTGGCTTAGCAGCA NGG 10 8 17 8 24 0 100 5 10
    240604234354_6dr8 TGGACCCCCAAAAGTTCATGCACTGACGGCTG(+ACTCAC)GATCCTCGGGCCGTAGAGTTCAGTGTAGGAT NGG 10 8 17 8 24 0 100 5 10
    240604234351_qipv CTCCCATCCTACACTGAACTCTACGGCCCGAG(+ACTCAC)GATCCAGCCGTCAGTGCATGAACTTTTGGGG NGG 10 8 17 8 24 0 100 5 10
    240604234350_eplg ATCCTGATTTAGAATAATTGACACTGTTTTAG(+ACTCAC)GTACCCGTTTTCTATTACCTTAAACTATTCA NGG 10 8 17 8 24 0 100 5 10
    240604234348_qp5b GATACTGAATAGTTTAAGGTAATAGAAAACGG(+ACTCAC)GTACCTAAAACAGTGTCAATTATTCTAAATC NGG 10 8 17 8 24 0 100 5 10