Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604234514_ibvy TAGGGCTTCTCTCCAGTGTGAGTCCTTTCATG(+ACTCAC)GATCCGGAAAGAATGAGAGCAACTGAAGGCC NGG 10 8 17 8 24 0 100 5 10
    240604234512_97jc CACAAGGCCTTCAGTTGCTCTCATTCTTTCCG(+ACTCAC)GATCCATGAAAGGACTCACACTGGAGAGAAG NGG 10 8 17 8 24 0 100 5 10
    240604234510_02v9 ACCACATGGTGGCTCACAACCATCTGTGATGG(+ACTCAC)GATCCAATGCCCTCTTCTGGTGTGTCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240604234509_dv7z CTTGTCTTCAGACACACCAGAAGAGGGCATTG(+ACTCAC)GATCCCATCACAGATGGTTGTGAGCCACCAT NGG 10 8 17 8 24 0 100 5 10
    240604234506_ae38 CTCATGGAAGCTATTTTGCTCTGTACCCCGGG(+ACTCAC)GATCCCCAGCATTAGGCCATTGGACCCCATC NGG 10 8 17 8 24 0 100 5 10
    240604234504_rhao AGAGAGATGGGGTCCAATGGCCTAATGCTGGG(+ACTCAC)GATCCCCGGGGTACAGAGCAAAATAGCTTCC NGG 10 8 17 8 24 0 100 5 10
    240604234503_xkao AAGCTAAATGTGTGCATACTCTATGTGGAGTG(+ACTCAC)GATCCACTCCGAACTGTACACCCTAAATACA NGG 10 8 17 8 24 0 100 5 10
    240604234501_5tzm GCACATGTATTTAGGGTGTACAGTTCGGAGTG(+ACTCAC)GATCCACTCCACATAGAGTATGCACACATTT NGG 10 8 17 8 24 0 100 5 10
    240604234459_e9kl GGGCTCTGCTCAGCATTGTCTGAGGGGTTGGG(+ACTCAC)GTACCCACACAGGTGCTTGCTGCTTCCCACG NGG 10 8 17 8 24 0 100 5 10
    240604234457_awum AATGCCGTGGGAAGCAGCAAGCACCTGTGTGG(+ACTCAC)GTACCCCAACCCCTCAGACAATGCTGAGCAG NGG 10 8 17 8 24 0 100 5 10