Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604234615_rlpu TTTCTTGAAAGGCTGAGAGAAGCACGGGGTTG(+ACTCAC)GTACCTTAATTTTCCAGCTGTTAAAGGAGAA NGG 10 8 17 8 24 0 100 5 10
    240604234614_fo8s TGCATTTCTCCTTTAACAGCTGGAAAATTAAG(+ACTCAC)GTACCAACCCCGTGCTTCTCTCAGCCTTTCA NGG 10 8 17 8 24 0 100 5 10
    240604234613_xq54 TCTTTATCCAGTGCCTGTGATAAACTCTCTGG(+ACTCAC)GATCCAGTGTCCACTTGGGGAAGGTGGGGCT NGG 10 8 17 8 24 0 100 5 10
    240604234611_guz9 AGCAGAGCCCCACCTTCCCCAAGTGGACACTG(+ACTCAC)GATCCCAGAGAGTTTATCACAGGCACTGGAT NGG 10 8 17 8 24 0 100 5 10
    240604234610_xo5m AATTTGGGTGGATGGGTTACTGCATTCTCAAG(+ACTCAC)GATCCATGGCATAGGAAATTGTTCTTCTGGG NGG 10 8 17 8 24 0 100 5 10
    240604234608_ykoy AACTACCCAGAAGAACAATTTCCTATGCCATG(+ACTCAC)GATCCTTGAGAATGCAGTAACCCATCCACCC NGG 10 8 17 8 24 0 100 5 10
    240604234606_r987 GGATGATTCTACCTCCAGACTTCCTCTTCCTG(+ACTCAC)GATCCATAGCAATATGCAGCTATGGCCTTGT NGG 10 8 17 8 24 0 100 5 10
    240604234605_g02k TTTATACAAGGCCATAGCTGCATATTGCTATG(+ACTCAC)GATCCAGGAAGAGGAAGTCTGGAGGTAGAAT NGG 10 8 17 8 24 0 100 5 10
    240604234603_vwo2 TTCCCTTCAGTATCTGCTCAGGCAGAAGATGG(+ACTCAC)GTACCCCCACCTCCACCATCATCTTCTGCTC NGG 10 8 17 8 24 0 100 5 10
    240604234601_8x08 CGTGGGAGCAGAAGATGATGGTGGAGGTGGGG(+ACTCAC)GTACCCATCTTCTGCCTGAGCAGATACTGAA NGG 10 8 17 8 24 0 100 5 10