Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604234559_jme1 ATGTTCTGGGATGCCTCCCCTGAAGCCAATGG(+ACTCAC)GTACCAACATCACCAGGAGGAGGAAGCAAAA NGG 10 8 17 8 24 0 100 5 10
    240604234558_x9td GACTCTTTTGCTTCCTCCTCCTGGTGATGTTG(+ACTCAC)GTACCCATTGGCTTCAGGGGAGGCATCCCAG NGG 10 8 17 8 24 0 100 5 10
    240604234556_pj8h AGTCTGTGAGGGAGCCTGACAGGGAGCACCAG(+ACTCAC)GATCCTTCACCTCCCCACTGCCGTGTGTCTA NGG 10 8 17 8 24 0 100 5 10
    240604234555_o6b5 GTGTGTAGACACACGGCAGTGGGGAGGTGAAG(+ACTCAC)GATCCTGGTGCTCCCTGTCAGGCTCCCTCAC NGG 10 8 17 8 24 0 100 5 10
    240604234553_aej0 AAGGTGAATCACAAGTATTGGAAACAGTTCAG(+ACTCAC)GATCCTGGCAGTAGCTTTCTCAGAGCAGCCT NGG 10 8 17 8 24 0 100 5 10
    240604234551_x7gk TGGGAAGGCTGCTCTGAGAAAGCTACTGCCAG(+ACTCAC)GATCCTGAACTGTTTCCAATACTTGTGATTC NGG 10 8 17 8 24 0 100 5 10
    240604234550_7m00 CCCAGCCAAGGAGCACGAGTGAGAACGCTCGG(+ACTCAC)GATCCGAGAGAAAAACAGGAGCAGCAGCACC NGG 10 8 17 8 24 0 100 5 10
    240604234549_frh5 TGGGAGGTGCTGCTGCTCCTGTTTTTCTCTCG(+ACTCAC)GATCCCGAGCGTTCTCACTCGTGCTCCTTGG NGG 10 8 17 8 24 0 100 5 10
    240604234547_60bu AACCAGGCTGGGATATGACACCTCCAGTGGAG(+ACTCAC)GATCCTTCTACACACAACACAGGTTTAGAAG NGG 10 8 17 8 24 0 100 5 10
    240604234545_tjip GTTTTCTTCTAAACCTGTGTTGTGTGTAGAAG(+ACTCAC)GATCCTCCACTGGAGGTGTCATATCCCAGCC NGG 10 8 17 8 24 0 100 5 10