Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604233316_j64o AGTGGCAGGGGTCAGCTTTCTCCATCATGTAG(+ACTCAC)GTACCAGATATCAAACCCAGGTTTTCAGTTT NGG 10 8 17 8 24 0 100 5 10
    240604233315_uugv TACCCAAACTGAAAACCTGGGTTTGATATCTG(+ACTCAC)GTACCTACATGATGGAGAAAGCTGACCCCTG NGG 10 8 17 8 24 0 100 5 10
    240604233314_r958 TGTGGGAATCAGTTCTCTTCTTCCAGCATGTG(+ACTCAC)GATCCTGGGACTTGAACTTAGGTCTTCAGGC NGG 10 8 17 8 24 0 100 5 10
    240604233312_e3yd CCATAGCCTGAAGACCTAAGTTCAAGTCCCAG(+ACTCAC)GATCCACATGCTGGAAGAAGAGAACTGATTC NGG 10 8 17 8 24 0 100 5 10
    240604233310_1fus AAGTATGCCAGCAGCAGACTCCTGAAGGTCAG(+ACTCAC)GATCCCTCACCTGAACTCCAAAACTATCAAA NGG 10 8 17 8 24 0 100 5 10
    240604233309_lvb4 AGGTCTTTGATAGTTTTGGAGTTCAGGTGAGG(+ACTCAC)GATCCTGACCTTCAGGAGTCTGCTGCTGGCA NGG 10 8 17 8 24 0 100 5 10
    240604233307_54jm CCTTGCTTACTGCGATCTATGAATAAACTAGG(+ACTCAC)GATCCTCCAAGGCAAACTGGATTGTTGCAAT NGG 10 8 17 8 24 0 100 5 10
    240604233305_if5n ATGTCATTGCAACAATCCAGTTTGCCTTGGAG(+ACTCAC)GATCCCTAGTTTATTCATAGATCGCAGTAAG NGG 10 8 17 8 24 0 100 5 10
    240604233304_8pod CCTTGCTTACTGCGATCTATGAATAAACTAGG(+ACTCAC)GATCCTCCAAGGCAAACTGGATTGTTGCAAT NGG 10 8 17 8 24 0 100 5 10
    240604233302_xwl7 ATGTCATTGCAACAATCCAGTTTGCCTTGGAG(+ACTCAC)GATCCCTAGTTTATTCATAGATCGCAGTAAG NGG 10 8 17 8 24 0 100 5 10