Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604233301_323y AGTGACAGGGTAGAATTCAGGCTCTTACATAG(+ACTCAC)GATCCAAGTTTCATCCATGGACAGGGAGTTC NGG 10 8 17 8 24 0 100 5 10
    240604233300_guam CCTCTGAACTCCCTGTCCATGGATGAAACTTG(+ACTCAC)GATCCTATGTAAGAGCCTGAATTCTACCCTG NGG 10 8 17 8 24 0 100 5 10
    240604233258_40hs TGTTTTAAAAATCAAATTCGGCTGAGCTGTTG(+ACTCAC)GATCCGGGTGACACAGGTGACAAGCAAGAGT NGG 10 8 17 8 24 0 100 5 10
    240604233256_dab7 AGGGGACTCTTGCTTGTCACCTGTGTCACCCG(+ACTCAC)GATCCAACAGCTCAGCCGAATTTGATTTTTA NGG 10 8 17 8 24 0 100 5 10
    240604233255_ji9c CTGAGCAGGCAAGCAGTCATCCCAAGAAGATG(+ACTCAC)GTACCCACTGTCCAGTTGACAGTCAATTTGG NGG 10 8 17 8 24 0 100 5 10
    240604233253_vv67 CAAAACCAAATTGACTGTCAACTGGACAGTGG(+ACTCAC)GTACCATCTTCTTGGGATGACTGCTTGCCTG NGG 10 8 17 8 24 0 100 5 10
    240604233252_1bve TTCTTATTTCAGTATTCCCAGAGCTAAGAATG(+ACTCAC)GTACCTAATATACAGTAAGCCCTCAATAAAT NGG 10 8 17 8 24 0 100 5 10
    240604233251_zgfe TGAGTATTTATTGAGGGCTTACTGTATATTAG(+ACTCAC)GTACCATTCTTAGCTCTGGGAATACTGAAAT NGG 10 8 17 8 24 0 100 5 10
    240604233249_4nfz TGAACCTAAGGCACAATAGGAAAGGGAGGTGG(+ACTCAC)GTACCAGTGTGGGTCACAGCCTGAAGCCTGT NGG 10 8 17 8 24 0 100 5 10
    240604233248_vxu5 GGGGTACAGGCTTCAGGCTGTGACCCACACTG(+ACTCAC)GTACCCACCTCCCTTTCCTATTGTGCCTTAG NGG 10 8 17 8 24 0 100 5 10