Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604233154_o3ej TCTACCCCATGTGGTCTGCCTCTGGTGTGGTG(+ACTCAC)GTACCTGGTCCTTTTTATACCCCTTCCACCT NGG 10 8 17 8 24 0 100 5 10
    240604233152_hebf AGCCAAGGTGGAAGGGGTATAAAAAGGACCAG(+ACTCAC)GTACCACCACACCAGAGGCAGACCACATGGG NGG 10 8 17 8 24 0 100 5 10
    240604233150_ep5g CCTTCTGCACTCAGCCTTTCCTTGGGGCTAGG(+ACTCAC)GATCCATCACATGGGGTAAGGCCTAGCCTAA NGG 10 8 17 8 24 0 100 5 10
    240604233149_xpbf TTGGATTAGGCTAGGCCTTACCCCATGTGATG(+ACTCAC)GATCCCTAGCCCCAAGGAAAGGCTGAGTGCA NGG 10 8 17 8 24 0 100 5 10
    240604233148_vmxl GGTGGGATGAGTACAGGGCCTTCAGGTCTCAG(+ACTCAC)GATCCCAAAGCCTCTGTTGCCCCATGGGGAT NGG 10 8 17 8 24 0 100 5 10
    240604233146_aruq TAGTCATCCCCATGGGGCAACAGAGGCTTTGG(+ACTCAC)GATCCTGAGACCTGAAGGCCCTGTACTCATC NGG 10 8 17 8 24 0 100 5 10
    240604233145_9gjn TTTTCTAAATATCATCAGTCCACATTTGGTTG(+ACTCAC)GATCCAGACATCCAGAGCTGGGGGTGCAGAG NGG 10 8 17 8 24 0 100 5 10
    240604233143_q21v CAGCCCTCTGCACCCCCAGCTCTGGATGTCTG(+ACTCAC)GATCCAACCAAATGTGGACTGATGATATTTA NGG 10 8 17 8 24 0 100 5 10
    240604233142_yi23 GGTGGGATGAGTACAGGGCCTTCAGGTCTCAG(+ACTCAC)GATCCCAAAGCCTCTGTTGCCCCATGGGGAT NGG 10 8 17 8 24 0 100 5 10
    240604233140_j5nk TAGTCATCCCCATGGGGCAACAGAGGCTTTGG(+ACTCAC)GATCCTGAGACCTGAAGGCCCTGTACTCATC NGG 10 8 17 8 24 0 100 5 10