Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604233121_6w7s TCTAAAAGATGAAATATCCACTATCCAAAGGG(+ACTCAC)GATCCTCAGAGGGCAACATAGTCAGCGACCA NGG 10 8 17 8 24 0 100 5 10
    240604233119_n61e AGGACTTTGACTAGCTGGAGAGGTCTGGGGAG(+ACTCAC)GATCCAGGGCAGCACAAATGGGATGAAGCTG NGG 10 8 17 8 24 0 100 5 10
    240604233116_ifnc CTTTGCAGCTTCATCCCATTTGTGCTGCCCTG(+ACTCAC)GATCCTCCCCAGACCTCTCCAGCTAGTCAAA NGG 10 8 17 8 24 0 100 5 10
    240604233115_3z9l TCACTGGCCAGTTAGCCTAAGCAAAGTAAATG(+ACTCAC)GTACCTAAAAGAACACATAAGGTTCCCCTCT NGG 10 8 17 8 24 0 100 5 10
    240604233114_i1sv GGGCCAGAGGGGAACCTTATGTGTTCTTTTAG(+ACTCAC)GTACCATTTACTTTGCTTAGGCTAACTGGCC NGG 10 8 17 8 24 0 100 5 10
    240604233112_ltyp GCTGGTACAGACTCCTATGGAGTTTTGCTGAG(+ACTCAC)GTACCAACCATGGGAGAGCACAAGATGTTTG NGG 10 8 17 8 24 0 100 5 10
    240604233111_tr3l CTCTCCAAACATCTTGTGCTCTCCCATGGTTG(+ACTCAC)GTACCTCAGCAAAACTCCATAGGAGTCTGTA NGG 10 8 17 8 24 0 100 5 10
    240604233109_6ggc GAGGATGCTCGGAGTGGCACCAGAGATGGCAG(+ACTCAC)GTACCACCCTGACTCTAGTTGTCTGTTGCTG NGG 10 8 17 8 24 0 100 5 10
    240604233107_9adn GTGAGCAGCAACAGACAACTAGAGTCAGGGTG(+ACTCAC)GTACCTGCCATCTCTGGTGCCACTCCGAGCA NGG 10 8 17 8 24 0 100 5 10
    240604233106_5vfv AGTGTGCTGTTGCCTATCTTTTTGACATCTGG(+ACTCAC)GTACCTGTGATGGCCTACAGGAAGCCCACAC NGG 10 8 17 8 24 0 100 5 10