Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604232952_gzxl AGTGAGATTTAGATCCGTAGTACTGAGGGTTG(+ACTCAC)GATCCAGAGTTTGTTACATGTGAGGGAAGAG NGG 10 8 17 8 24 0 100 5 10
    240604232950_vydw TTTCGGGGTCTCTGTTTCTTTGTCTATAAAAG(+ACTCAC)GATCCTTTACTAGGATAGAAAGTTGTGATAT NGG 10 8 17 8 24 0 100 5 10
    240604232949_4s82 AAAACATATCACAACTTTCTATCCTAGTAAAG(+ACTCAC)GATCCTTTTATAGACAAAGAAACAGAGACCC NGG 10 8 17 8 24 0 100 5 10
    240604232947_x23n GGAAGGCCATGCTGTCCACCCTTCCTGGCCAG(+ACTCAC)GATCCAGTTGAGCAGCAGAGGTTGACTAAAT NGG 10 8 17 8 24 0 100 5 10
    240604232945_gm2h CCTTCATTTAGTCAACCTCTGCTGCTCAACTG(+ACTCAC)GATCCTGGCCAGGAAGGGTGGACAGCATGGC NGG 10 8 17 8 24 0 100 5 10
    240604232944_5b0r TATGGTTAGGTCTTCAGAACTTAATTAAATTG(+ACTCAC)GATCCTGGAGATGAGGCACATGGGTAGAGAA NGG 10 8 17 8 24 0 100 5 10
    240604232942_u3xh CTACTTTCTCTACCCATGTGCCTCATCTCCAG(+ACTCAC)GATCCAATTTAATTAAGTTCTGAAGACCTAA NGG 10 8 17 8 24 0 100 5 10
    240604232941_qio9 GCTACCCTTAACTTTCACAGATCTAATAATCG(+ACTCAC)GATCCCTAAAATAAACTTGAGGCACATTTTG NGG 10 8 17 8 24 0 100 5 10
    240604232938_905o ATGTTCAAAATGTGCCTCAAGTTTATTTTAGG(+ACTCAC)GATCCGATTATTAGATCTGTGAAAGTTAAGG NGG 10 8 17 8 24 0 100 5 10
    240604232936_y8xo CAAACTGCTGCCACTCTACAAGCAAATGCCTG(+ACTCAC)GATCCCTTCTGATGTGCAGTAACCAGGACTC NGG 10 8 17 8 24 0 100 5 10