Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604232935_dg3a AAGGTGAGTCCTGGTTACTGCACATCAGAAGG(+ACTCAC)GATCCAGGCATTTGCTTGTAGAGTGGCAGCA NGG 10 8 17 8 24 0 100 5 10
    240604232932_oita TGTGCCTCAAAGTTCGGTTGGGGCTACCTTGG(+ACTCAC)GATCCCAAACAGCTAATATGGTCACCAACTG NGG 10 8 17 8 24 0 100 5 10
    240604232931_9qj7 ATAGCCAGTTGGTGACCATATTAGCTGTTTGG(+ACTCAC)GATCCCAAGGTAGCCCCAACCGAACTTTGAG NGG 10 8 17 8 24 0 100 5 10
    240604232929_517u CTCTGGAGACATCTTCAGCCTGAGGAGGCTGG(+ACTCAC)GTACCAAGGAGGAAGAACCCAGCTGGCTTTA NGG 10 8 17 8 24 0 100 5 10
    240604232927_r5sd GGTGGTAAAGCCAGCTGGGTTCTTCCTCCTTG(+ACTCAC)GTACCCAGCCTCCTCAGGCTGAAGATGTCTC NGG 10 8 17 8 24 0 100 5 10
    240604232925_pj98 ATCCCTCAGGCTCTGCTGTGGACTAGTTCCAG(+ACTCAC)GATCCCTGTCACCAAGTATATGAAAACCTGA NGG 10 8 17 8 24 0 100 5 10
    240604232923_8olx CATCCTCAGGTTTTCATATACTTGGTGACAGG(+ACTCAC)GATCCTGGAACTAGTCCACAGCAGAGCCTGA NGG 10 8 17 8 24 0 100 5 10
    240604232921_oweo GTTTAAAAAAAGAAAAAAAGAAAAAAAAACAG(+ACTCAC)GTACCTGAAATCCCAGCACTTGAGAGGTTGA NGG 10 8 17 8 24 0 100 5 10
    240604232920_slaj CTGGCTCAACCTCTCAAGTGCTGGGATTTCAG(+ACTCAC)GTACCTGTTTTTTTTTCTTTTTTTCTTTTTT NGG 10 8 17 8 24 0 100 5 10
    240604232919_yxt0 TGACCAGCCAGCCACCCTAGCCTACTTGTTGG(+ACTCAC)GTACCAGGCCAATGGGAAAGCCTGTCTCAAA NGG 10 8 17 8 24 0 100 5 10